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Paper Information

Journal:   JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT)   SPRING 2006 , Volume 32 , Number 1 (SECTION: BIOLOGY 1); Page(s) 29 To 35.
 
Paper: 

EXPRESSION OF THE MOUSE G α Q USING BACUFOVIRUS EXPRESSION SYSTEM

 
 
Author(s):  ARAB NAJAFI S.M.*
 
* SCHOOL OF BIOLOGICAL SCIENCES, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Baculovirus expression system has been used successfully to over-express eukaryotic proteins in insect cells. This system uses a very strong viral promoter, AcNPV polyhedrin, for high level protein expression. Here a Baculovirus expression system was used to express the Mouse Gaq protein (mGaq) in SF9 insect cells. The recombinant protein was made at high levels and it was found in the cell membrane where it functions as a signaling molecule.

 
Keyword(s): BACULOVIRUS, G-PROTEINS, EXPRESSION SYSTEM, INSECT CELLS
 
References: 
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