Paper Information

Journal:   MODARES JOURNAL OF MEDICAL SCIENCES (PATHOBIOLOGY)   SPRING 2006 , Volume 9 , Number 1; Page(s) 57 To 66.
 
Paper: 

EXVIVO EVALUATION OF CANDIDA ALBICANS INFECTED MACROPHAGES AND NEUTROPHILS FUNCTION OF DIABETIC RATS

 
 
Author(s):  MOGHTADAEI M., ZAVARAN HOSSEINI A.*, YADGARI M.H.
 
* DEPARTMENT OF IMMUNOLOGY, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Patients afflicted by diabetes mellitus (DM) usually have more infections than those without DM. The course of the infections is also more complicated in this group of patients. One of the possible causes of increased infections prevalence is a deficiency in the immunity. Besides some decreased cellular responses in vitro, no disturbances in adaptive immunity in diabetic patients have been described. Different disturbances (low complement factor 4, decreased cytokine response after stimulation) in humoral innate immunity have been described in diabetic patients. In this research hydrogen peroxide (reactive oxygen mediator) and nitric oxide (reactive nitrogen mediator) in the neutrophil and macrophage culture of peritone in rats were evaluated against C.albicans. Via intravenous injection of streptozocin (65 mg/kg), a diabetic rat model was obtained. nitric oxide and hydrogen peroxide assay was performed by the Griess and Walter-Ruch methods respectively. C.albicans colony count on SCC medium was also done in two groups of healthy and diabetic mice.
Macrophages of the healthy group reacted to C.albicans severly compared to the diabetic group which significantly produced more nitric oxide (p
£0.028). Neutrophils of the healthy group produced more No compared to the diabetic group against C.albicans (P£0.165). No considerable difference was observed in production of hydrogen peroxide by macrophages in two groups of healthy and diabetic mice. Neutrophils of the diabetic group produced more hydrogen peroxide compared to the healthy group (P£1). There was no significant difference in C.albicans colony count between the two healthy and diabetic groups (P£0.058).
Although nitrogen and oxygen related factors are changed or reduced after diabetic induction, changes in other immune system factors cannot be undermined.

 
Keyword(s): DIABETES, NEUTROPHIL, MACROPHAGE, NITRIC OXIDE, HYDROGEN PEROXIDE, RAT
 
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