Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCES   2006 , Volume 12 , Number 1 (SUPPLEMENT); Page(s) 175 To 185.
 
Paper: 

A STUDY ON THE EFFECT OF GROWTH REGULATORS AND AMMONIUM TO NITRATE RATIO ON IN VITRO CULTURE OF MINIATURE ROSE

 
 
Author(s):  HASHEMI MOGHADAM H., KHALIFA A., ZIARAT NIA M.
 
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Abstract: 

Experiments were conducted to study the proliferation and rooting ability of Miniature rose. Single-node explants were sterilized and cultured on MS medium supplemented with various concentrations of BA and NAA. Also in different stages, ammonium to nitrate ratios in combination to hormones for number and length of shoot were used different. Combination of 0/5 mg/l BA with 0/05 mg/l NAA was the best treatment for establishment stage. Combination of 0/5 mg/l BA with 0/01- 0/1 mg/l NAA was optimal concentration for multiplication stage. In other establishment experiment optimal ratio of ammonium to nitrate and BA was 37/63 and 1 mg/l for proliferation and shoot length respectively. In the multiplication stage, ammonium to nitrate 0/100 without GA3 for shoot length was the optimal treatment. For rooting higher root formation and the number of roots was obtained using 30 mM Nitrogen than 60 mM. Quick-dip method using 500-mg/l NAA in vitro showed better rooting than other NAA concentrations. Effect of Ammonium to nitrogen on rooting was not significant.

 
Keyword(s): MINIATURE ROSE, MICRO PROPAGATION, HORMONE, AMMONIUM TO NITRATE RATIO
 
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