Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCES   2006 , Volume 12 , Number 1 (SUPPLEMENT); Page(s) 87 To 96.
 
Paper: 

EFFECTS OF HEAT STRESS ON POLLEN GERMINATION AND TUBE GROWTH IN MAIZE (INVITRO CONDITION)

 
Author(s):  NAJAFI E., REZA NADVAR O.
 
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Abstract: 

In order to investigate the effect of six different tempertures on percent of pollen germination and tube length in 17 inbred lines in corn (Zea maize), an experiment was carried out in 2001. To produce the necessary pollens, the lines were planted in three plantin date. pollens were collected between 9-10 a.m. Freshly collected pollen was placed in in 6x1.5 cm petri plates contain modified nutrient medium developed by Pfahler (1976) and Fraleigh (1985), and then immediately exposed to different temperature treatments.percent of pollen germination was investigated in 200 pollen using 5 microscope. tube length was measured in 20 pollen. results showed that some of the lines had greater resistance against heat tension. the best temperture for pollen germination was less than the temperture necessary for tube enlargement. also the difference between pollen germination and tube length in different lines, showed that these characteristics are genetically controled make it possible to produce resistant hybrids. in this study line number 11 showed the greatest percent of pollen germination and the line number 8 showed the longest tube length. according to the results the best temperture for germination was 20 C° while the best temperture for tube growth was 25 Co.

 
Keyword(s): HEAT TENSION, POLLEN, CORN
 
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