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Paper Information

Journal:   IRANIAN JOURNAL OF BIOTECHNOLOGY   APRIL 2007 , Volume 5 , Number 2; Page(s) 114 To 118.
 
Paper: 

BIOSORPTION OF TEXTILE DYES AND EFFLUENTS BY PLEUROTUS FLORIDA AND TRAMETES HIRSUTA WITH EVALUATION OF THEIR LACCASE ACTIVITY

 
 
Author(s):  MOORTHI PERUMAL S.*, MUNUSWAMY D., SELLAMUTHU P.S., KANDASAMY M., THANGAVELU K.P.
 
* DEPARTMENT OF INDUSTRIAL BIOTECHNOLOGY, DR. M.G.R. EDUCATIONAL AND RESEARCH INSTITUTE, DR. M.G.R. UNIVERSITY, CHENNAI-600 095, INDIA
 
Abstract: 

The rate and efficiency of decolorization of dyes like Blue CA, Black B133, and Corazol Violet SR were tested to evaluate white rot fungal strains. Trametes hirsute and Pleurotus florida showed the greatest extent of decolorization on nutrient salt media. Maximum decolorization of 200 mg/l of Blue 133 was obtained by 4 days old incubated Pleurotus florida followed by Trametes hirsuta after 6 days. An attempt was made to improve the decolorization activity of both organisms with different concentrations of glucose 1 and 2% (w/v). The decolorization activity may be due to the laccase enzyme of white rot fungi. The production of this enzyme was estimated using solid state fermentation with rice bran as a substrate. It was found that P. florida exhibited 0.175 U/ml of laccase activity followed 0.126U/ml by T. hirsute, respectively. Decolourization was found to be more effective with P. florida in the presence of 2% (w/v) glucose. Crude extract containing the laccase enzyme was isolated and confirmed by SDS PAGE.

 
Keyword(s): DYE HOUSE EFFLUENT, LACCASE, PLEUROTUS FLORIDA, REACTIVE TEXTILE DYES, TRAMETES HIRSUTA
 
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