Paper Information

Journal:   IRANIAN JOURNAL OF BIOTECHNOLOGY   APRIL 2007 , Volume 5 , Number 2; Page(s) 110 To 113.
 
Paper: 

PRODUCTION AND PURIFICATION OF A PROTEASE FROM AN ALKALOPHILIC BACILLUS SP. 2-5 STRAIN ISOLATED FROM SOIL

 
 
Author(s):  FALAHAT PISHEH H.R.*, KHOSRAVI DARANI KIANOUSH, JALALI M., BADAMI N., MARDANI N.
 
* DEPARTMENT OF FOOD TECHNOLOGY RESEARCH, NATIONAL NUTRITION AND FOOD TECHNOLOGY RESEARCH INSTITUTE, SHAHID BEHESHTI UNIVERSITY, M.C., TEHRAN, I.R. IRAN
 
Abstract: 

This research has focused on isolation and characterization of a strain of Bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at pHs ranging from 8 to 11 and temperatures of 20 to 50°C. Also the impact of different carbon and nitrogen sources were investigated. The yield and fold of enzyme purification was 24% and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline protease produced by Bacillus sp. 2 - 5 showed the most caseinolytic activity (without any gelatinolytic activity) at pH>10.

 
Keyword(s): ALKALOPHILIC BACILLUS, PROTEASE, PROCESS VARIABLES, PURIFICATION
 
References: 
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