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Paper Information

Journal:   JOURNAL OF DENTAL SCHOOL SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCE   SPRING 2007 , Volume 25 , Number 1 (71); Page(s) 18 To 25.
 
Paper: 

DIAGNOSTIC VALUE OF HUMAN TELOMERASE FOR DETECTION OF SQUAMOUS CELL CARCINOMA IN ORAL SMEARS

 
 
Author(s):  BAGHERI A.*, ESLAMI B., KAZEMI B., MOSHREF M., AZARGASHB E.A., SABA N.
 
* DEPT. OF ORAL AND MAXILLOFACIAL PATHOLOGY, DENTAL SCHOOL, GILAN UNIVERSITY OF MEDICAL SCIENCES RASHT, IRAN
 
Abstract: 

Background & Aim: Human telomerase has been associated with most malignant tumors such as squamous cell carcinoma (SCC) of the oral cavity. One simple non-aggressive method for early diagnosis of oral SCC is taking smears. The aim of this study was to assess the frequency of telomerase expression in smears of oral SCC, compared with normal mucosa.

Methods & Materials: Smears were obtained from 18 subjects with SCC of different sites in the oral cavity, presenting with various degrees of malignancy. Control samples were taken from the contra-lateral normal epithelium of the same subjects. The expression of hTERT, the most reliable marker of telomerase activity, was registered by extracting RNA, performing RT-PCR and then nested PCR by specific primers. Fisher's exact test was used to analyze the data.

Results: Positive expression of telomerase was detected in 8 of the 18 cases, while the normal epithelial samples demonstrated no such expression of telomerase. A significant difference in the expression of telomerase was reported between the two groups by Fisher's Exact test (P<0.003).

Conclusion: There is a potential for telomerase enzyme activity to act as a simple non-aggressive diagnostic tool in detection of oral SCC using smears of the lesion.

 
Keyword(s): ORAL SQUAMOUS CELL CARCINOMA, TELOMERASE ENZYME, SMEAR
 
References: 
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