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Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ)   2007 , Volume 17 , Number 2; Page(s) 135 To 143.
 
Paper: 

MICROSATELLITES POLYMORPHISM OF GENE BOLA-DRB3 IN SARABI AND HOLSTEIN BREEDS AND ITS ASSOCIATION WITH MASTITIS DISEASE

 
 
Author(s):  FIROUZ AMANDI M.*, SHOJAA J., JABARPOUR BONYADI MORTEZA, LEGHAIEAN N., GHORBANI A.
 
* DEPARTMENT OF ANIMAL SCIENCE, FACULTY OF AGRICULTURE, UNIVERSITY OF TABRIZ, TABRIZ, IRAN
 
Abstract: 

One of the immune system genes which has important role in mastitis disease is BoLA-ORB3. Initially, 30 Sarabi cows (from Sarab region and around) and 39 Holstein cows (from several herds of Tehran region) were selected. Then 214 blood samples from Holstein cattle (Mogan Plant Production & Industry Corporation) were provided. Out of these 214 cattle, 150 cattle were susceptible and 64 cattle were resistant to mastitis (within 41 half-sib families). In order to study microsatellite polymorphism in intron 2 of BoLA-DRB gene and to obtain allelic frequency and to compare allelic variability between Sarabi and Holstein cows, all blood samples were randomly collected. In these samples two microsatellite markers ORB3 and DRBP1 were studied (the two microsatellites DRBP1 and ORB3 are located in intron 2 of the corresponding DRB3 gene). In the second stage, microsatellite marker DRB3 was analyzed in 214 of Holstein cows for studying probable linkage between disease and different alleles of BoLA-DRB3 gene. In the Sarabi breed, 9 and 5 different alleles and in Holstein breed 10 and 4 different alleles were observed for DRB3 and DRBP1 microsatellites, respectively. The amount of heterozygosity for two loci in the Sarabi and Holstein breeds were estimated as 0.49 and 0.67, respectively. Association between mastitis and microsatellite DRB3 alleles was studied among 214 Holstein cows. Statistically significant (P<0.05) association between allele 5 of DRB3 microsatellite and mastitis disease was observed. The results of this study demonstrate that DRB3 and DRBP1 microsatellites are highly polymorphic in the Iranian native Sarabi cattle and can be used as genetic markers. In this study, close association was found between one of the several alleles of DRB3 microsatellite and mastitis.

 
Keyword(s): MASTITIS DISEASE, MICROSATELLITE BOLA-DRB3 AND DRBP1, POLYMORPHISM, SARABI AND HOLSTEIN BREED
 
References: 
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