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Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ)   2007 , Volume 17 , Number 2; Page(s) 109 To 115.
 
Paper: 

ASSESSMENT OF DIRECT REGENERATION POTENTIAL OF THREE COTTON CULTIVARS THROUGH SHOOT APEX CULTURE

 
 
Author(s):  GAROUSI SH.*, TOUHIDFARD MASOUD, RAHIMIAN H., KAZEMI TABAR S.K., NEMATZADEH GH.A.
 
* FACULTY OF AGRICULTURE, GILAN UNIVERSITY, RASHT, IRAN
 
Abstract: 

Progress of an effective, genotype-independent regeneration system is crucial to gain any advances in the field of cotton (Gossypium hirsutum L.) gene transfer and biotechnology. The aim of this research was to study regeneration potential of shoot apex explants. Shoot apices from Sahel, Varamin and Coker 312 cultivars were cut and transferred to MS basal medium containing 100 mg/l of myo-inositol and 0.5 mg/l of each of nicotinic acid, pyridoxine and thiamine. Following several subcultures and removal of whitened explants, regenerated shoots were transferred to previous medium (without growth regulators) for root induction. After root development and acclimatization, they were maintained in greenhouse. Results showed that there were no significant differences between three Sahel, Varamin and Coker 312 cultivars concerning survived shoot apex percentage and regenerated plantlets. A total percentage of 53.9 of excised shoot apices belonging to three cultivars were regenerated and survived. Plantlets were successfully established in pots with a mean of 3.9 percent. Frequency of plantlets with abnormal phenotypes was so low (0.69%). No calli were observed at the shooting period and just one unique shoot was developed from each shoot apex explants. According to results, use of shoot apex explants could be so appropriate for tissue culture and genetic engineering of Iranian cotton cultivars.

 
Keyword(s): BIOTECHNOLOGY, GENOTYPE, GOSSYPIUM HIRSUTUM L, REGENERATION, SHOOT APEX
 
References: 
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