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Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ)   2007 , Volume 17 , Number 1; Page(s) 151 To 163.
 
Paper: 

DETECTION OF POLYMORPHISM BY RAPD-PCR IN LOCAL POPULATIONS OF SWEETPOTATO WHITEFLY IN IRAN

 
 
Author(s):  SAMIE M.A.*, KAMALI K., TALEBI ALI ASGHAR, JALALI JAVARAN MOKHTAR
 
* DEPT. OF PLANT PROTECTION, COLLEGE OF AGRICULTURE, UNIVERSITY OF VALIE ASR, RAFSANJAN, IRAN
 
Abstract: 

The sweetpotato whitefly, Bemisia tabaci(Genn.) (Hom: Aleyrodidae) is a major pest of field crops, vegetables and omamentals in Iran. Economic losses caused by the pest may be through viral transmission, excretion of honeydew or by direct damage to the plants. The infested leaves containing nymphs and pupae were collected from cotton fields in Darab, Qom, Saveh, Gonbad, Gorgan, Varamin, Garmsar, College of Agriculture, Orsoiieh and Rafsanjan (Kerman) and Shooshtar. Experiments were conducted on cotton Gossypium hirsutum L. (Varamin 76 variety) in a growth chamber under 24±2°C, 55±3% RH and 16:8 (L: D) photoperiod. Random amplified polymorphic DNA was used to detect genetic diversity in 11 Bemisia tabaci populations in 2001. In this study five decamer primers were used in the amplification reactions. All the primers produced polymorphisms. All together 71 RAPD markers were found. The amplified DNA fragments were in the range of 200 to 3000 bp. The Jaccards and Nei's similarity coefficient and Euclidean distances were used to generatea dendrogrambased on unweightedpair - group method with arithmetic averages (UPGMA). The cluster analysis divided the genotypes into six clusters. The clusters were at 1) Darab 2) Shooshtar 3) Orsoiieh genotype 4) Rafsanjan 5) Varamin 6) Garmsar, Saveh, College of Agriculture, Qom, Gorgan and Gonbad. To confirm the above phonogram, cluster analysis based on biological traits using Euclidean distance method and the UPGMA algorithm was used. It was found that both techniques produced similar results. In both cases, no relationship was observed between genetic and geographical diversity. The clusters based on RAPD markers correlated fairly well with classification scheme based on biological traits. This study suggested that RAPD can be a useful technique to study DNA polymorphism in Bemisia tabaci.

 
Keyword(s): BEMISIA TABACI, COTTON WHITEFLY, GENETIC DIVERSITY, MOLECULAR MARKERS, RAPD- PCR
 
References: 
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