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Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   SUMMER 2007 , Volume 6 , Number 3; Page(s) 179 To 184.
 
Paper: 

THE EFFECT OF DIFFERENT STABILIZERS ON STABILITY OF HORSERADISH PEROXIDASE- BOVINE SERUM ALBUMIN-AFLATOXIN B1, A CONJUGATED TRACER FOR DETECTION OF AFLATOXIN B1 IN IMMUNOASSAY-BASED METHODS

 
 
Author(s):  FARZAMFAR B., BAYAN ALHAGH S., MAHBOUDI F., ZAHRAEI M.*
 
* DEPARTMENT OF MEDICAL BIOCHEMISTRY, SCHOOL OF MEDICINE, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. ELISA is one of the sensitive methods for detection of aflatoxins. Preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an ELISA method. In current study, different stabilizers were applied to stabilize a newly prepared conjugated molecule, Horseradish peroxidase-Bovine serum albumin-Aflatoxin B1 (HRP-BSA-AFB1). Stabilizing effects of six different stabilizers were compared during 10 months verification at room temperature and 6 weeks verification at 37°C. Based on the results, it was concluded that trehalose-containing stabilizers especially those contain casein in their compositions show the best stabilizing effects on HRP-BSA-AFB1 conjugated tracer.

 
Keyword(s): AFLATOXIN, BSA, CONJUGATE, HRP, IMMUNOASSAY, STABILIZER
 
References: 
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