Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)   SUMMER 2007 , Volume 14 , Number 2 (SN 44); Page(s) 16 To 22.
 
Paper: 

ISOLATION AND PRIMARY CULTURE OF RAT HEPATOCYTES USING KIWIFRUIT ACTINIDIN

 
 
Author(s):  SHIRVANI FARSANI Z., MANSOURI K., BIDMESHKIPOUR A., MOSTAFAEI ALI*
 
* MEDICAL BIOLOGY CENTER, KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES & HEALTH SERVICES, KERMANSHAH, IRAN
 
Abstract: 

Introduction & Objective: Isolation of cells from different tissues rely on proteolytic enzymes mainly collagenases that selectively digest collagen fibers of extra-cellular matrix. It is important to find new and proper collagenases from plant sources. In the present research actinidin, a cysteine protease abundant in Kiwifruit, was used to isolate and culture of rat hepatocytes.
Materials & Methods: Different concentrations of actinidin was used to isolate rat hepatocytes by one or two-step perfusion method. The viability of the separated cells was examined by the trypan blue test. The isolated rat hepatocytes were cultured on collagen coated plates in William's E medium. The morphology of hepatocytes was examined microscopically after staining with the Papanicolaou method.
Results: Actinidin in the concentration of 0.4 mg/ml in two-step perfusion method properly isolated hepatocytes from rat liver. The viability of isolated hepatocytes that successfully cultured in collagen coated plates was 90-95 percent.
Conclusion: These results showed that actinidin is a proper protease for isolation of hepatocytes. In addition, purification of this enzyme is simpler than the collagenases.

 
Keyword(s): ACTINIDIN, COLLAGENASE, HEPATOCYTE
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 63
 
Latest on Blog
Enter SID Blog