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Paper Information

Journal:   PHARMACEUTICAL SCIENCES   SUMMER 2007 , Volume - , Number 2; Page(s) 31 To 38.
 
Paper: 

EFFECT OF ZINC, SELENIUM AND COPPER IN CELL LINE OF BREAST CANCER ON TELOMERASE GENE EXPRESSION

 
 
Author(s):  ZARGHAMI N.A.*, ASADI J.
 
* DRUG APPLID RESEARCH CENTER, TABRIZ UNIVERSITY OF MEDICAL SCIENCES
 
Abstract: 

Objectives: Telomerase is a RNA dependent DNA polymerase which synthesis telomeric repeats and in most cases are molecular base of unlimited proliferation. Telomerase gene expression occurs in most tumor cells rather than normal cells. Combination of external and internal factors including Zn, Cu and Selenium are involved in breast tumor development and growth. The aim of this study was to evaluate the effect of Zinc, Selenium and Copper on telomerase gene expression in breast cancer T47D cell line.
Methods: Time and dose dependent manner treatment of T47D breast cancer cell lines were carried out by adding Zn, Cu, and Selenium in tissue culture. Relative telomerase activity (RTA) of T47D cell line was measured by TRAP assay.
Results: After being treated with 100
mM/L Zn So4 for 6 hours, telomerase activity of T47D cells was markedly increased (5.2 fold) but with 100 mM /L Zn So4 for 24 hours and 500 mM /L Zn So4 for 6, 24 hours, telomerase activity was 0.76, 0.39 and 0.12% respectively (control 49.2%). After being treated with 10 mM /L Cu So4 for 6 hours, telomerase activity of T47D cells 3.67 fold was increased. After being treated with 10, 30 mM /L selenium-L- methuonin, telomerase activity were markedly inhibited. Telomerase activity of T47D cells for 24 hours were 0.93, 0.60 and for 48 hours were 0.76, 0.12 respectively (control 49.2%).
Conclusion: Based on obtained results, it is speculated that pharmacologic doses of Se, Zn and Cu could have an inhibitory effects on telomerase gene expression and it could be used as a target therapy in tumor cells.

 
Keyword(s): BREAST CANCER, ZN, CU, SE AND TELOMERASE
 
 
References: 
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Click to Cite.
APA: Copy

ZARGHAMI, N., & ASADI, J. (2007). EFFECT OF ZINC, SELENIUM AND COPPER IN CELL LINE OF BREAST CANCER ON TELOMERASE GENE EXPRESSION. PHARMACEUTICAL SCIENCES, -(2), 31-38. https://www.sid.ir/en/journal/ViewPaper.aspx?id=101317



Vancouver: Copy

ZARGHAMI N.A., ASADI J.. EFFECT OF ZINC, SELENIUM AND COPPER IN CELL LINE OF BREAST CANCER ON TELOMERASE GENE EXPRESSION. PHARMACEUTICAL SCIENCES. 2007 [cited 2021May08];-(2):31-38. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=101317



IEEE: Copy

ZARGHAMI, N., ASADI, J., 2007. EFFECT OF ZINC, SELENIUM AND COPPER IN CELL LINE OF BREAST CANCER ON TELOMERASE GENE EXPRESSION. PHARMACEUTICAL SCIENCES, [online] -(2), pp.31-38. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=101317.



 
 
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