Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   JOURNAL OF VETERINARY RESEARCH   2003 , Volume 58 , Number 3; Page(s) 287 To 291.
 
Paper: 

COMPARISON OF THREE METHODS: CULTURE OF FECES, AGGLUTINATION TEST AND DOT ELISA FOR DIAGNOSIS OF INAPPARENT INFECTION OF SALMONELLA DUBLIN IN CALVES

 
 
Author(s):  ZAHRAEI SALEHI M.T.*, NADALIAN M.GH., FAGHIH HABIBI Y.
 
* DEPARTMENT OF MICROBIOLOGY AND IMMUNOLOGY, FACULTY OF VETERINARY MEDICINE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Objective: Diagnosis of Salmonella carrier calves by using feces culture, agglutination test and Dot ELISA.
Procedure: 216 sera and feces samples were obtained from calves in farms of around of Tehran. The feces samples were cultured in enrichment and selective media and then isolated Salmonella were serotyped by O and H antisera. The sera samples were tested for O and H agglutinins via Widal and Dot ELISA tests.
Statistical analysis: ANOVA and when a significance different was seen, Duncan's Multiple Range Test.
Results: In this study two serotypes including: Salmonella typhimurium (4 cases) and Salmonella dublin (2 cases) were isolated from feces. In serological tests 5 and 15 sera samples were positive in Widal and ELISA tests respectively.
Conclusion: From the results of this study it seems that dot Elisa method is very sensitive in diagnosis of. Salmonella carrier calves than other tests.

 
Keyword(s): SALMONELLOSIS, WIDAL TEST, DOT ELISA, CATTLE, CARRIER, SALMONELLA DUBLIN
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 75
 
Latest on Blog
Enter SID Blog