Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST)   FALL 2007 , Volume 4 , Number 4 (16); Page(s) 513 To 520.
 
Paper: 

BIOSYNTHESIS OF BIODEGRADABLE POLYHYDROXYALKANOTES BIOPOLYMERS IN GENETICALLY MODIFIED YEASTS

 
 
Author(s):  ABD EL HALEEM D.*, AMARA A., ZAKI S., ABULREESH G., ABULHAMD A.
 
* ENVIRONMENTAL BIOTECHNOLOGY DEPARTMENT AND PROTEIN RESEARCH DEPARTMENT, GENETIC ENGINEERING AND BIOTECHNOLOGY RESEARCH INSTITUTE (GEBRI), MUBARAK CITY FOR SCIENTIFIC RESEARCH AND TECHNOLOGY APPLICATIONS, NEW BURG-ELARAB CITY, ALEXANDRIA, EGYPT
 
Abstract: 
In the recent decade, biosynthesis of the degradable biopolymers polyhydroxyalkanotes in transgenic yeasts became an important research task. Most research strategies depend on either metabolic engineering or molecular approaches. In the present work, research compared PHA biosynthesis in two types of yeasts; Saccharomyces cerevisiae and a non-convenient Kloeckera spp. Yeast strains were equipped in their cytoplasm with the phaABCRe operon containing genes phbA, phbB and phbC of the PHA biosynthetic pathway of Ralstonia eutropha, which encode β-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively. The transgenic strains Saccharomyces cerevisiae and Kloeckera sp. were able to produce PHA. The maximum content of the polymer detected in the recombinant strain INVSc1/PHA1 was 2.68 % and only poly-3-hydroxybutyrate (PHB) accumulated. However, the non-conventional transgenic strain KY1/PHA was able to accumulate as maximum of 7.06 % of the copolymer poly-(3-hydroxybutyrateco- poly-3-hydroxyvalerate) (PHV). Western blot analysis confirmed expression of the phaABCRe operon in the transgenic yeast strains. The nature of the PHA thus produced by all tested strains was analyzed by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy.
 
Keyword(s): RECOMBINANT YEASTS, POLYHYDROXYALKANOTES, POLY-3-HYDROXYBUTYRATE, NUCLEAR MAGNETIC RESONANCE
 
 
References: 
 
Citations: 
  • Not Registered.
 
+ Click to Cite.
APA: Copy

ABD EL HALEEM, D., & AMARA, A., & ZAKI, S., & ABULREESH, G., & ABULHAMD, A. (2007). BIOSYNTHESIS OF BIODEGRADABLE POLYHYDROXYALKANOTES BIOPOLYMERS IN GENETICALLY MODIFIED YEASTS. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST), 4(4 (16)), 513-520. https://www.sid.ir/en/journal/ViewPaper.aspx?id=87696



Vancouver: Copy

ABD EL HALEEM D., AMARA A., ZAKI S., ABULREESH G., ABULHAMD A.. BIOSYNTHESIS OF BIODEGRADABLE POLYHYDROXYALKANOTES BIOPOLYMERS IN GENETICALLY MODIFIED YEASTS. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST). 2007 [cited 2021December03];4(4 (16)):513-520. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=87696



IEEE: Copy

ABD EL HALEEM, D., AMARA, A., ZAKI, S., ABULREESH, G., ABULHAMD, A., 2007. BIOSYNTHESIS OF BIODEGRADABLE POLYHYDROXYALKANOTES BIOPOLYMERS IN GENETICALLY MODIFIED YEASTS. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST), [online] 4(4 (16)), pp.513-520. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=87696.



 
  pdf-File
Yearly Visit 71
 
 
Latest on Blog
Enter SID Blog