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Journal:   JOURNAL OF CROP BIOTECHNOLOGY   winter 2019 , Volume 8 , Number 24 ; Page(s) 71 To 93.
 
Paper: 

Study Morpho-Physiological Change And Protein Pattern Of Tolerant And Susceptible Wheat Cultivars Under Salinity Stress By Using Proteome Analysis

 
 
Author(s):  Naghavi Mohammad Reza, Khalili Marouf*
 
* Department of Agriculture, Payame Noor University, Tehran, Iran
 
Abstract: 
In order to investigate the mechanism of tolerance to salt stress in wheat, a factorial experiment basis of completely randomized design with four replications in greenhouse media were conducted. The factors of this experiment were included salinity of sodium chloride in four levels of zero (control), 100, 200 and 300 mM and two wheat cultivars namely Niknejhad and Pishtaz as a representative of tolerant and susceptible cultivars, respectively. Leaf samples were prepared two weeks after the starting of salt stress. Then, extraction of protein from leaf tissue was done and two-dimensional electrophoresis in control plants and under salt stress plants were carried out. The results of proteome analysis revealed that 15 replicated protein spots with different expression variations were common between two tolerant and susceptible cultivars, and five protein spots of unique to each tolerant and susceptible cultivar, had significantly expression variations under stress. Protein spots were detected by mass spectrometry and the results showed that more number from commonly detected proteins were classified in functional groups including antioxidant defense and Calvin cycle, while other proteins in each cultivar had more roles in antioxidant activity. Overall, the results showed that there was a significant difference between two cultivars in terms of morpho-physiological response to salt stress, and the Niknejhad cultivar showed a better protein response under stress.
 
Keyword(s): Proteome analysis,responsive proteins to stress,tolerance of salt stress,wheat
 
 
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APA: Copy

NAGHAVI, M., & KHALILI, M. (2019). Study morpho-physiological change and protein pattern of tolerant and susceptible wheat cultivars under salinity stress by using proteome analysis. JOURNAL OF CROP BIOTECHNOLOGY, 8(24 ), 71-93. https://www.sid.ir/en/journal/ViewPaper.aspx?id=757364



Vancouver: Copy

NAGHAVI MOHAMMAD REZA, KHALILI MAROUF. Study morpho-physiological change and protein pattern of tolerant and susceptible wheat cultivars under salinity stress by using proteome analysis. JOURNAL OF CROP BIOTECHNOLOGY. 2019 [cited 2022May29];8(24 ):71-93. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=757364



IEEE: Copy

NAGHAVI, M., KHALILI, M., 2019. Study morpho-physiological change and protein pattern of tolerant and susceptible wheat cultivars under salinity stress by using proteome analysis. JOURNAL OF CROP BIOTECHNOLOGY, [online] 8(24 ), pp.71-93. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=757364.



 
 
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