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Paper Information

Journal:   JOURNAL OF REPRODUCTION AND INFERTILITY   APRIL-JUNE 2019 , Volume 20 , Number 2; Page(s) 95 To 101.
 
Paper: 

Evaluation of Sperm Chromatin Integrity Using Aniline Blue and Toluidine Blue Staining in Infertile and Normozoospermic Men

 
 
Author(s):  Pourmasumi Soheila, KHORADMEHR AREZOO, Rahiminia Tahereh, SABETI PARVIN, Talebi Ali Reza*, Ghasemzadeh Jalal
 
* Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
 
Abstract: 
Background: Male infertility is defined as a man lost his ability to fertilize a fertile female naturally. Diagnosis of male infertility cannot be made just according to basic semen analysis. It is necessity to have specific tests for evaluation of chromatin in-tegrity. In this study, an attempt was made to evaluate the sperm chromatin quality in fertile men and infertile subgroup. Methods: Among 1386 couples, 342 men were categorized into normospermia and 1044 were infertile and they were referred to Yazd Research and Clinical Center for infertility treatment. Standard semen analysis and sperm nuclear maturity tests in-cluding aniline blue (AB) and toluidine blue (TB) staining were done. Data were an-alyzed by SPSS software. The p≤ 0. 05 was considered statistically significant. Results: The mean value of TB staining was significantly higher in infertile group compared to normospermic group (p=0. 005). Mean of sperm normal morphology was lower in idiopathic infertile men in comparison with normozoospermic men (p= 0. 001). The highest negative correlation was obtained between sperm count and AB staining. Progressive motility was negatively correlated with AB and TB staining in both groups but there was no significant difference between AB staining and pro-gressive motility in men normospermia group. Conclusion: Sperm chromatin staining using AB and TB showed a negative associa-tion between sperm chromatin condensation with sperm count, normal morphology and progressive motility. It seems that the AB and TB test may be useful for the as-sessment of male fertility potential.
 
Keyword(s): Aniline blue,Infertility,Sperm,Toluidine blue
 
 
References: 
 
Citations: 
 
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APA: Copy

POURMASUMI, S., & KHORADMEHR, A., & RAHIMINIA, T., & SABETI, P., & TALEBI, A., & GHASEMZADEH, J. (2019). Evaluation of Sperm Chromatin Integrity Using Aniline Blue and Toluidine Blue Staining in Infertile and Normozoospermic Men. JOURNAL OF REPRODUCTION AND INFERTILITY, 20(2), 95-101. https://www.sid.ir/en/journal/ViewPaper.aspx?id=695761



Vancouver: Copy

POURMASUMI SOHEILA, KHORADMEHR AREZOO, RAHIMINIA TAHEREH, SABETI PARVIN, TALEBI ALI REZA, GHASEMZADEH JALAL. Evaluation of Sperm Chromatin Integrity Using Aniline Blue and Toluidine Blue Staining in Infertile and Normozoospermic Men. JOURNAL OF REPRODUCTION AND INFERTILITY. 2019 [cited 2021August02];20(2):95-101. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=695761



IEEE: Copy

POURMASUMI, S., KHORADMEHR, A., RAHIMINIA, T., SABETI, P., TALEBI, A., GHASEMZADEH, J., 2019. Evaluation of Sperm Chromatin Integrity Using Aniline Blue and Toluidine Blue Staining in Infertile and Normozoospermic Men. JOURNAL OF REPRODUCTION AND INFERTILITY, [online] 20(2), pp.95-101. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=695761.



 
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