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Journal:   JOURNAL OF BIOTECHNOLOGY   Spring-SUMMER 2013 , Volume 4 , Number 1 ; Page(s) 57 To 64.
 
Paper: 

Increased The Genes Expression Of Phenylpropanoid Biosynthesis Pathway And Production Of Podophyllotoxin By Chitosan In Cell Cultures Of Linum Album

 
 
Author(s):  Esmaeilzadeh Bahabadi Sedigheh, Sharifi Mozafar, Behmanesh Mehrdad
 
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Abstract: 
Linum album is an herbaceous and medicinal plant that has important lignan such as podophyllotoxin (PTOX). PTOX has antiviral and anticancer properties. Since the chemical synthesis of PTOX is an expensive process، production of PTOX using cell and cultures of linum species is a cost-effective alternative approach. Various strategies have been employed to increase the production of secondary metabolites in cell cultures. In this study، we have verified the effect of chitosan on cell growth، PTOX production in 1، 2، 3 and 5 days after treatment. Cells elicited with chitosan for 5 days yielded the highest amount of PTOX. To study mechanism of chitosan action، expression of phenylalanine ammonio-lyase (PAL)، cinnamoyl-CoA reductase (CCR)، cinnamyl alcohol dehydrogenase (CAD) and pinoresinol lariciresinol reductase (PLR) genes were investigated. The expression of genes were increased، reaching a peak at 3 day after treatment. Chitosan up-regulate the production of PTOX، by effecting on gene expression of PTOX biosynthesis pathway.
 
Keyword(s): gene expression,podophyllotoxin,chitosan and linum album
 
 
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APA: Copy

ESMAEILZADEH BAHABADI, S., & SHARIFI, M., & BEHMANESH, M. (2013). Increased the genes expression of phenylpropanoid biosynthesis pathway and production of podophyllotoxin by chitosan in cell cultures of Linum album. JOURNAL OF BIOTECHNOLOGY, 4(1 ), 57-64. https://www.sid.ir/en/journal/ViewPaper.aspx?id=659971



Vancouver: Copy

ESMAEILZADEH BAHABADI SEDIGHEH, SHARIFI MOZAFAR, BEHMANESH MEHRDAD. Increased the genes expression of phenylpropanoid biosynthesis pathway and production of podophyllotoxin by chitosan in cell cultures of Linum album. JOURNAL OF BIOTECHNOLOGY. 2013 [cited 2022May29];4(1 ):57-64. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=659971



IEEE: Copy

ESMAEILZADEH BAHABADI, S., SHARIFI, M., BEHMANESH, M., 2013. Increased the genes expression of phenylpropanoid biosynthesis pathway and production of podophyllotoxin by chitosan in cell cultures of Linum album. JOURNAL OF BIOTECHNOLOGY, [online] 4(1 ), pp.57-64. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=659971.



 
 
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