Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)   Spring 2000 , Volume 11 , Number 1; Page(s) 35 To 41.
 
Paper: 

Molecular Weight Dtermlnation And Acetylcholinesterase Using Sucrose Gradient

 
 
Author(s):  Elmi Akhoni S.*
 
* Tehran University of Medical Sciences
 
Abstract: 
Molecular weight determination of proteins especially enzymes are the most routine and popular method in Enzmology. For molecular weight and its subunits determination, a homogenous wistar Rat Brain was prepared in a phosphate buffer. After centrifugation, supernatant which contained, "soluble Enzyme" was isolated. The second stage, pellet was brought to the original volume with phosphate buffer contained Triton X-100 with a certain concentration.After second Centrifugation in a certain RPM, the supernatant which is called "detergent soluble Enzyme" was isolated. At the same time Sucrose Gradient in the plastic tubes were prepared and applied on to the top Gradient and centrifused for over night. The next day all plastic tubes were put on the stand and samples were prepared through a small hole made in the buttom of this plastic tubes and finally the Enzyme activity were determined.
 
Keyword(s): ENZYME, ACETYLCHOLINESTERASE, SUCROSE GRADIENT
 
 
International related papers: 
 
Most related Highly related Moderately related Least related
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
  • Not Registered.
 
+ Click to Cite.
APA: Copy

ELMI AKHONI, S. (2000). MOLECULAR WEIGHT DTERMLNATION AND ACETYLCHOLINESTERASE USING SUCROSE GRADIENT. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 11(1), 35-41. https://www.sid.ir/en/journal/ViewPaper.aspx?id=57881



Vancouver: Copy

ELMI AKHONI S.. MOLECULAR WEIGHT DTERMLNATION AND ACETYLCHOLINESTERASE USING SUCROSE GRADIENT. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES). 2000 [cited 2022January27];11(1):35-41. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=57881



IEEE: Copy

ELMI AKHONI, S., 2000. MOLECULAR WEIGHT DTERMLNATION AND ACETYLCHOLINESTERASE USING SUCROSE GRADIENT. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), [online] 11(1), pp.35-41. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=57881.



 
 
Persian Abstract Yearly Visit 35
 
 
Latest on Blog
Enter SID Blog