Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES   JUNE-JULY 2016 , Volume 38 , Number 2 ; Page(s) 72 To 79.
 
Paper: 

TARGETING OF M13 PHAGE PARTICLES BY HUMAN HOLOTRANSFERRIN USING A CHEMICAL COUPLING STRATEGY

 
 
Author(s):  KAVOOCI MAHBOUBEH, KHALAJ KONDORI MOHAMMAD*, KADIVAR MEHDI
 
* DEPARTMENT OF ANIMAL SCIENCES, SCHOOL OF NATURAL SCIENCE, UNIVERSITY OF TABRIZ, TABRIZ, IRAN
 
Abstract: 
Background & Objectives: Targeting transgene carriers and vectors to individual cells and tissues is one of the most important goals of gene therapy. Bacteriophages are of appropriate transgene carriers and there are different methods for their targeting to target cells. Present study reports preparation of targeted M13-based bacteriophage particles by a chemical coupling strategy.Materials and Methods: First, the pCMV-Script-GFP construct was produced via in vivo excision protocol from l-GFP Phage particles using ExAssist helper phage and XLOLR as specific host. Then, M13 phage particles bearing GFP (M13-GFP) were obtained by single stranded rescue using R408 helper phage. The human holotransferrin molecules were then coupled to the surface of phage particles by reductive amination chemistry. Transferrin molecules bind to the surface of phage particles were studied by phage-ELISA.Results: Phage-ELISA tests showed that holotransferrin molecules were coupled to the surface of M13 phage particles in a correct way and the transferrin-targgeted M13 phage particles were prepared. Further analysis showed that about 485 transferrin molecules coupled per phage particle.Conclusion: The results show that chemical coupling might be considered as a suitable strategy for targeting of M13 particles via coupling of targeting molecules in high density to the phage surface.
 
Keyword(s): CHEMICAL COUPLING, M13 PHAGE, HOLOTRANSFERRIN, TARGETING
 
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
  • Not Registered.
 
+ Click to Cite.
APA: Copy

KAVOOCI, M., & KHALAJ KONDORI, M., & KADIVAR, M. (2016). TARGETING OF M13 PHAGE PARTICLES BY HUMAN HOLOTRANSFERRIN USING A CHEMICAL COUPLING STRATEGY. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 38(2 ), 72-79. https://www.sid.ir/en/journal/ViewPaper.aspx?id=567931



Vancouver: Copy

KAVOOCI MAHBOUBEH, KHALAJ KONDORI MOHAMMAD, KADIVAR MEHDI. TARGETING OF M13 PHAGE PARTICLES BY HUMAN HOLOTRANSFERRIN USING A CHEMICAL COUPLING STRATEGY. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES. 2016 [cited 2021December04];38(2 ):72-79. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=567931



IEEE: Copy

KAVOOCI, M., KHALAJ KONDORI, M., KADIVAR, M., 2016. TARGETING OF M13 PHAGE PARTICLES BY HUMAN HOLOTRANSFERRIN USING A CHEMICAL COUPLING STRATEGY. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, [online] 38(2 ), pp.72-79. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=567931.



 
 
Persian Abstract Yearly Visit 44
 
 
Latest on Blog
Enter SID Blog