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Paper Information

Journal:   JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY   2017 , Volume 3 , Number 1; Page(s) 42 To 52.
 
Paper: 

ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE

 
 
Author(s):  RASULI ELNAZ, TAFVIZI FARZANEH*, TAJABADY EBRAHIMI MARYAM
 
* DEPARTMENT OF BIOLOGY, PARAND BRANCH, ISLAMIC AZAD UNIVERSITY, PARAND, IRAN
 
Abstract: 

Aopoptosis induction is one of the best method cancer therapy and funding the anti-cancer component, especially inducing opoptosis is of the significant important. This study aimed to assess cytotoxic effects of heat-killed probiotic bacteria and induced apoptosis on gastric cancer AGS cells compared to normal cells (HEK-293). AGS cancer cell line AGS was cultured in RPMI medium, and cell line HEK-239 was selected as the control. Both cell lines received 0.01, 0.1, 1, 10, 100, and 1000 mg/ml of heat-killed lactobacillus paracasei for periods of 24, 48, and 72 hours. Viability of cells was measured using MTT assay. Apoptosis assay was performed in IC50 of cell culture by Annexin V and propidium iodide staining methods in 48 hours. According to MTT assay, heat-killed bacteria reduced viability and proliferation of gastric cancer cell line AGS in pattern of time, dose and strain dependent. The highest cytotoxic effect was observed in 1000 mg/ml for 72 hours. Annexin V staining and flow cytometry confirmed induced apoptosis in AGS cell line in pattern of time, dose and strain dependent. Heat-killed probiotic bacteria showed cytotoxic and induced apoptosis effects on AGS gastric cancer cell line, with slight cytotoxic effect on HEK-239 compared to AGS. Further studies are still required to confirm these bacteria as a biological anti-cancer product in treatment and prevention.

 
Keyword(s): CYTOTOXIC, GASTRIC CANCER HEAT-KILLED PROBIOTIC BACTERIA
 
 
References: 
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Cite:
APA: Copy

RASULI, E., & TAFVIZI, F., & Tajabady Ebrahimi, m. (2017). ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE. JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY, 3(1), 42-52. https://www.sid.ir/en/journal/ViewPaper.aspx?id=545321



Vancouver: Copy

RASULI ELNAZ, TAFVIZI FARZANEH, Tajabady Ebrahimi maryam. ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE. JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY. 2017 [cited 2021April23];3(1):42-52. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=545321



IEEE: Copy

RASULI, E., TAFVIZI, F., Tajabady Ebrahimi, m., 2017. ANTIPROLIFERATION EFFECT OF HEAT KILLED INDIGENOUS PROBIOTIC BACTERIA ON AGS CELL LINE. JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY, [online] 3(1), pp.42-52. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=545321.



 
 
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