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Paper Information

Journal:   JOURNAL OF VETERINARY RESEARCH   2016 , Volume 71 , Number 4; Page(s) 431 To 436.
 
Paper: 

IDENTIFICATION OF CAMPYLOBACTER SPP. FROM POULTRY SKIN USING METHODS BASED ON BACTERIAL CULTURE AND POLYMERASE CHAIN REACTIONS

 
 
Author(s):  EBRAHIMI LAGHA F.*, ZEYNALI F., REZAZADEH BARI M.
 
* FACULTY OF AGRICULTURE, UNIVERSITY OF URMIA, URMIA, IRAN
 
Abstract: 

BACKGROUND: Food infections caused by Campylobacter are one of the gastrointestinal inflammations in humans is health and economic losses in the community is important.
OBJECTIVES: To determine the prevalence of Campylobacter contamination in chicken skin samples of Urmia, using bacterial culture and polymerase chain reactions.
METHODS: 80 samples of chicken skin from the Protein Gostare Sina slaughter house located in the city of Urmia in equal numbers in the winter and spring seasons were collected.
The survival of Campylobacter after 24 hours in refrigerated conditions was studied in samples. Positive samples were used for DNA extraction and PCR. To investigate the phylogenetic isolates, positive samples PCR were sequenced.
RESULTS: 58.75% of chicken skin using bacterial cultures, Campylobacter were positive. The Results study the survivalCampylobacter in cold conditions after 24 hours, showed that no significant decrease in the survivalCampylobacter as well as contamination levels were significantly higher in spring than in winter, which may be due to the high temperature of environment that created the favorable conditions for Campylobacter.
CONCLUSIONS: Chicken skin is the reservoir of Campylobacter. This issue of public health care and control at all stages of production and supply of poultry products, also the transfer of it to other parts of poultry carcasses should be considered.

 
Keyword(s): CHICKEN SKIN, CAMPYLOBACTER, BACTERIAL CULTURES, POLYMERASE CHAIN REACTION
 
References: 
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