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Paper Information

Journal:   SADRA MEDICAL SCIENCES JOURNAL   SPRING 2016 , Volume 4 , Number 2; Page(s) 127 To 135.
 
Paper: 

IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR

 
 
Author(s):  GANJIPOUR M., AMINI K.*, MORADLI G.
 
* DEPARTMENT OF MICROBIOLOGY, SCHOOL OF BASIC SCIENCES, SAVEH BRANCH, ISLAMIC AZAD UNIVERSITY, SAVEH, IRAN
 
Abstract: 

Background: Shigella is responsible for shigellosis or bacillary dysentery, which is transmitted by contaminated food. Invasion Plasmid Antigens (Ipa) gene leads to intra- or extra-cellular spreading of Shigella. The current study aimed to identify Ipa and stx genes in Shigella sonnei isolated from clinical samples by multiplex-PCR and determine their antibiotic resistance profile.
Methods: In this study, 150 stool samples were collected from different treatment centers in Tehran. After culture-based microbiological and biochemical tests, 60 Shigella sonnei species were identified. Antibiotic susceptibility tests were performed by disk diffusion method according to CLSI guideline. Then, multiplex-PCR assay was employed to identify stx and ipa genes.
Results: The results showed that the highest and lowest resistance was related to co-trimoxazole (n=60, 100%) and ceftriaxone (n=10, 16.7%), respectively. Out of the 60 isolates, 57 strains (95%) were positive for ipaH gene and other virulence genes were not detected in any of the samples.
Conclusion: ipaH, due to presence in the majority of Shigella strains, can be a candidate to identify this organism. It seems that indiscriminate use of antibiotics is one of the causes of antibiotic resistance in Shigella species.

 
Keyword(s): IPA GENE, STX GENE, SHIGELLA SONNEI, MULTIPLEX-PCR, ANTIBIOTIC RESISTANCE
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

GANJIPOUR, M., & AMINI, K., & MORADLI, G. (2016). IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR. SADRA MEDICAL SCIENCES JOURNAL, 4(2), 127-135. https://www.sid.ir/en/journal/ViewPaper.aspx?id=511000



Vancouver: Copy

GANJIPOUR M., AMINI K., MORADLI G.. IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR. SADRA MEDICAL SCIENCES JOURNAL. 2016 [cited 2021October23];4(2):127-135. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511000



IEEE: Copy

GANJIPOUR, M., AMINI, K., MORADLI, G., 2016. IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR. SADRA MEDICAL SCIENCES JOURNAL, [online] 4(2), pp.127-135. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511000.



 
 
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