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Paper Information

Journal:   JOURNAL OF CELL & TISSUE   FALL 2013 , Volume 4 , Number 3; Page(s) 287 To 295.
 
Paper: 

STUDY OF EFFECT OF COCL2 ON GROWTH PARAMETERS AND EXPRESSION OF ACC OXIDASE GENE IN POTATO (SOLANUM TUBEROSUM L.) CULTIVAR WHITE DESIREE, UNDER IN VITRO CULTURE

 
 
Author(s):  TAGHIZADEH MARZIEH, EHSANPOUR ALIAKBAR*
 
* DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCES, UNIVERSITY OF ISFAHAN, ISFAHAN, IRAN
 
Abstract: 
Aim: Ethylene, a phytohormone, is produced during in vitro plant tissue culture and its accumulation is associated with reduction of growth and morphological changes. The aim of this study was to evaluate application of CoCl2, as ethylene synthesis inhibitor, on growth and some physiological parameters as well as expression of ACC oxidase gene of potato.Material and Methods: In this study, nodal segments of potato (Solanum tuberosum L.) cv. White Desiree were cultured on MS medium containing concentration of 0, 5, 10, 15, 20, 30, 40, 60 mg/L CoCl2. After 5 weeks, chlorophyll a, b, total, cartenoied, content, leaf area and expression level of ACC oxidase gene were investigated.Results: CoCl2 inhibited root production in aerial parts and increased leaf areas and amount of chlorophyll a, b and carotenoid. Application of 20 mg/L CoCl2 was the best for potato growth. ACC oxidase gene expression was decreased at this concentration.Conclusion: Application of CoCl2 decreased ACC oxidase gene expression and prevented ethylene accumulation in the in vitro culture and consequently improved growth of potato plant.
 
Keyword(s): ACC OXIDASE (AMINOCYCLOPROPANE 1-CARBOXYLIC ACID), COCL2, ETHYLENE, IN VITRO CULTURE, POTATO
 
 
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APA: Copy

TAGHIZADEH, M., & EHSANPOUR, A. (2013). STUDY OF EFFECT OF COCL2 ON GROWTH PARAMETERS AND EXPRESSION OF ACC OXIDASE GENE IN POTATO (SOLANUM TUBEROSUM L.) CULTIVAR WHITE DESIREE, UNDER IN VITRO CULTURE. JOURNAL OF CELL & TISSUE, 4(3), 287-295. https://www.sid.ir/en/journal/ViewPaper.aspx?id=490024



Vancouver: Copy

TAGHIZADEH MARZIEH, EHSANPOUR ALIAKBAR. STUDY OF EFFECT OF COCL2 ON GROWTH PARAMETERS AND EXPRESSION OF ACC OXIDASE GENE IN POTATO (SOLANUM TUBEROSUM L.) CULTIVAR WHITE DESIREE, UNDER IN VITRO CULTURE. JOURNAL OF CELL & TISSUE. 2013 [cited 2021December03];4(3):287-295. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=490024



IEEE: Copy

TAGHIZADEH, M., EHSANPOUR, A., 2013. STUDY OF EFFECT OF COCL2 ON GROWTH PARAMETERS AND EXPRESSION OF ACC OXIDASE GENE IN POTATO (SOLANUM TUBEROSUM L.) CULTIVAR WHITE DESIREE, UNDER IN VITRO CULTURE. JOURNAL OF CELL & TISSUE, [online] 4(3), pp.287-295. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=490024.



 
 
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