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Paper Information

Journal:   IRANIAN JOURNAL OF BIOLOGY   Fall-Winter 2001-2002 , Volume 11 , Number 3-4; Page(s) 49 To 60.
 
Paper: 

SELECTION OF SALT TOLERATNT CALLUS IN MEDICAGO SATIVA L. CV. REHNANI

 
 
Author(s):  AMINI F., EHSANPOUR A.A.
 
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Abstract: 

Medicago is known as a relatively salt tolerant plant. The level of salt tolerance can be increased by callus culture and selection. Sterile seed were cultured and then their Root, stem and leaf segments cultured for callus production in MS medium with 2,4-D,NAA, Kin and Yeast extract. After one month callus production from the best explants occurred on the best medium. The experiment carried out in 2 ways. Calli were transferred directly to 120 mM NaCl and in the step method in 30, 60, 90, 120 150 and 180 mM NaCl. After 3 subcultures the final fresh weight of calli were measured. After 3 time subculture in salt free medium selected and unselected were transferred in media with 0, 30, 60, 90, 120, 150 and 180 mM NaCl. After 3 subcultures the final fresh weight as well as the pattern of SDS-PAGE. Results indicated that, the best medium for callus production was MS+2, 4-D (mgL-1). NAA (2mgL-1), Kin (2mgL-1) YE (1grL-1) and the best explants was stem. The rate of tolerance and the growth of the calli in the stepwise selection were better than direct way. Test of salt tolerance stability in the calli showed stable tolerance for salt stress. It can be consider as genetic or epigenetic changes.

 

 
Keyword(s): SALT TOLERANCE, TISSUE CULTURE, MEDICAGO SATIVA
 
References: 
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