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Paper Information

Journal:   KOWSAR MEDICAL JOURNAL   Summer 2005 , Volume 10 , Number 2; Page(s) 79 To 88.
 
Paper: 

EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS

 
 
Author(s):  MEHRANI H.A.*, SMALL D.
 
* Department of Biochemistry, and Research Center for Chemical Injuries, Faculty of Medicine, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, IR IRAN
 
Abstract: 
Objective: The aim of the present study was to examine the mechanism by which β-amyloid interact with nicotinic receptors and changes the level of cetylcholinesterase (AChE) in Alzheimer's disease. Materials and Methods: SHSY5Y and PC12 human neuroblastoma and  pheochromocytoma cells were cultured and effects of A β, and nicotinic receptor agonists and antagonists were  investigated. Results: A β 1-42 significantly increased AChE in SHSY5Y cells. The effect of A β was blocked by nicotine and α-bungarotoxin (α -Bgt) a selective antagonist of α 7 nicotinic acetylcholine receptor (nAChR). Nicotine alone had no effect on AChE activity, but acetylcholine, choline and carbamoylcholine all increased AChE activity in these cells. Nicotinic receptor antagonists such as α -Bgt, methellycaconitine (MLA), ecamylamine and dihydro β -erythroidin (DH β E) did not have any effect on AChE activity. In contrast, selective α 7 nAChR antagonist's α -Bgt and MLA in concomitant with 10 µM nicotine, significantly increased AChE activity in SHSY5Y cells, but non- α 7 preferring antagonist, DH β E was not effective. This activation was not seen in PC12 cells. Acetylcholine (but not nicotine) increased α 7 nAChR density in SHSY5Y cell membrane. Conclusion: These results demonstrate that increase in AChE activity in these cells is due to α 7 nAChR.    
 
Keyword(s): ACETYLCHOLINE, ACETYLCHOLINESTERASE, Α 7-NICOTINIC RECEPTOR, AMYLOID Β, SHSY5Y AND PC12 CELLS
 
 
References: 
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Citations: 
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APA: Copy

MEHRANI, H., & SMALL, D. (2005). EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS. KOWSAR MEDICAL JOURNAL, 10(2), 79-88. https://www.sid.ir/en/journal/ViewPaper.aspx?id=46552



Vancouver: Copy

MEHRANI H.A., SMALL D.. EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS. KOWSAR MEDICAL JOURNAL. 2005 [cited 2021December09];10(2):79-88. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=46552



IEEE: Copy

MEHRANI, H., SMALL, D., 2005. EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS. KOWSAR MEDICAL JOURNAL, [online] 10(2), pp.79-88. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=46552.



 
 
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