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Paper Information

Journal:   JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES   FALL 2015 , Volume 17 , Number 3 (55); Page(s) 114 To 121.
 
Paper: 

DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE

 
 
Author(s):  ESFANDIARI P., AMANI J.*, IMANI FOOLADI A.A., FORGHANIFARD M.M., MIRHOSSEINI S.A.
 
* APPLIED MICROBIOLOGY RESEARCH CENTER, BAQIYATALLAH UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background and Objective: Enterotoxigenic Escherichia coli (ETEC) are the most common agent which causes diarrhea, worldwide. ETEC is colonized along the cells and then producing heat-labile (LT) and heat-stable enterotoxigenic which enter into intestinal epithelial cells and causes water and electrolyte loss from intestinal epithelial cells and eventually cause diarrhea.This study was done to detect the heat-labile toxin in Enterotoxigenic Escherichia coli using PCR-ELISA technique.
Methods: In this descriptive study, DIG-labeled PCR products were bounded to streptoavidin-coated wells of a microtiter plate and detected by anti-DIG–peroxidase conjugate. The biotin-labeled internal probe was used for verification of PCR products.
Results: Heat-labile toxin was detected by PCR-ELISA method. The sensitivity of heat-labile toxin was 1.9 ng. This method did not cross-react with bacteria from this variety.
Conclusion: PCR-ELISA method is 100 times more sensitive than conventional PCR method and due to lack of agarose gel and electrophoresis device it can be a good alternative to traditional method.

 
Keyword(s): ENTEROTOXIGENIC ESCHERICHIA COLI, PCR, ELISA, HEAT, LABILE ENTEROTOXIN
 
 
References: 
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Citations: 
 
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APA: Copy

ESFANDIARI, P., & AMANI, J., & IMANI FOOLADI, A., & FORGHANIFARD, M., & MIRHOSSEINI, S. (2015). DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 17(3 (55)), 114-121. https://www.sid.ir/en/journal/ViewPaper.aspx?id=463591



Vancouver: Copy

ESFANDIARI P., AMANI J., IMANI FOOLADI A.A., FORGHANIFARD M.M., MIRHOSSEINI S.A.. DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES. 2015 [cited 2021October26];17(3 (55)):114-121. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=463591



IEEE: Copy

ESFANDIARI, P., AMANI, J., IMANI FOOLADI, A., FORGHANIFARD, M., MIRHOSSEINI, S., 2015. DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, [online] 17(3 (55)), pp.114-121. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=463591.



 
 
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