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Paper Information

Journal:   NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL   WINTER 2015 , Volume 5 , Number 17; Page(s) 69 To 73.
 
Paper: 

IN VITRO EVALUATION OF EFFECT OF SODIUM CHLORIDE ON STABILITY OF CYTOTOXICITY PROPERTY CISPLATINUM IN DIMETHYL SULFOXIDE SOLVENT ON G-292 CELL LINE

 
 
Author(s):  BAGHERPOUR DOUN SEYED KAZEM, AKBARZADEH AZIM*, HALALKHOR SOHRAB, EBRAHIMI SHAHMABADI HASAN, ALAVI SEYED EBRAHIM, MORTAZAVI MEHRI, SAFFARI ZAHRA, FARAHNAK MARYAM
 
* 
 
Abstract: 

Introduction: Cisplatin (Cispt) is an anti-cancer drug with a low level of solubility. One of Cispt’s solvents is dimethyl sulfoxide (DMSO) which can be substituted with chlorine of drug as Cispt’s solvent. Using this solvent is impossible in biological studies due to intense reduction in activity. On the other hand, it is specified that Cispt’s stability is increased in aqueous media by increasing sodium chloride (NaCl) concentration up to 0.9%. Consequently, we intended to study the effect of DMSO on cytotoxicity of Cispt in the presence of sodium.
Materials and Methods: G-292 cells and MTT assay was used to study cytotoxicity of different compounds in culture media. FTIR was employed to investigate chemical structure of Cispt and Cispt dissolved in DMSO.
Results: cytotoxicity in dilutions of 300 and 9 (p<0.01) of Cispt in Cispt+NaCl+DMSO formulation was equal to 78 and 7%. These numbers were estimated 79 and 18% for Cispt+ DMSO formulation. Studying chemical structure of Cispt and Cispt dissolved in DMSO showed that sodium chloride cannot inhibit inactivating effect of DMSO on Cispt and effect of this solvent on Cispt is independent from presence of sodium chloride.
Conclusion: This study showed that effect of solvent on Cispt is independent from presence of sodium chloride. Our findings suggest more studies to use this solvent for Cispt.

 
Keyword(s): DIMETHYL SULFOXIDE, SODIUM CHLORIDE, CISPLATIN, CYTOTOXICITY
 
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