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Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES   Spring 2003 , Volume 10 , Number 1; Page(s) 65 To 72.
 
Paper: 

CLASSIFICATION SEVERAL PUMPKIN VARIETIES BASED ON SEED TOTAL PROTEIN

 
 
Author(s):  SIAMI A., HEIDARI R., DASTPAK A.
 
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Abstract: 

The amount of total protein in seed from four varieties of Cucurbita was identified. Three varieties of these plants (ghalami, mashhadi, kaghazi) were from Cucurbita pepo and one variety (tanbal) was from Cucurbita maxima. Result showed the total protein range between 36.47-37.60%. Isolation and analysis of seed storage protein was carried out by Lamelli method and electrophoresis with polyacrylamid gel on SDS. Electrophorgram from seed storage protein showed 22 protein bands which their molecular masses were between 3.73-156 Kd and 12 protein bands are the same. Dendrogram of these 22 protein bands and similarity matrix showed similarity percent and genetically distance among them. First cluster included three varieties of C.pepo and second cluster included C.maxima, which had very low similarity in regard to first cluster and had genetically distance with three varieties of C.pepo. These result indicate that seed storage protein can be used to identify the genetically distance among varieties.

 

 
Keyword(s): STORAGE PROTEIN, ELECTROPHORESIS, CUCURBITA
 
References: 
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