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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   Summer 2003 , Volume 5 , Number 18; Page(s) 97 To 101.
 
Paper: 

CYTOGENETIC EFFECTS OF METHOTREXATE AND CYTARABINE ALONE AND IN COMBINATION ON G1 AND G2 CELL CYCLE STAGES OF LEUKEMIC MOLT-4 CELL LINE IN VITRO

 
 
Author(s):  BARADAR BOKAIE P.*, MOZDARANI H.
 
* 
 
Abstract: 

Introduction: This study was performed to analyze chromosomal aletrations in G1 and G2 immortalized MOLT-4 cell line following treatment with methotrexate (MTX) and cytarabine (ara-C) alone or incombination.

 

Material and Methods: MOLT-4 cells were cultured and manitained in RPMI-1640 supplementedwith fetal calf serum, antibiotics and L-gluthamine. Cells were treated with ara-C and MTX with a concentration of 100µmol/L at G1 and 50µmol/L at G2. One and half hour prior to harvesting, cell wereexposed to colcemid. (0.1µg/ml find concentration). Microscopic slides were prepared using standard1000).‚procedure. After staining with 5% Giemsa, metaphase was evaluated using light microscope (×1000).

 

Results: Results indicate that ara-C and MTX, at a 100µmol/L concentration, used for treatment of G1cells, increase the frequency of chromosomal type aberrations; although ara-C was more potent in aberrationinduction. The effect of combined treatment with ara-C and MTX on G1 cells was more than effect of eachdrug when used alone. The effect of these drugs on G2 cells with a concentration of 50µmol/L was found to besignificantly different with that of controls (P<0.05). The frequency of chromatid type breaks induced by ara-C was considerably higher than MTX. When these drug were uaed in combination , the frequencuy of aberration increased dramatically.

 

Conclusion: Results indicate that both ara-C and MTX produce clastogenic effects on MOLT-4 cells.Combination treatment with ara-C and MTX has an additive effect on G1-cells. But a synergistic effect wasfound when they were used for treatment of G2-cells. The reason for hypersensitivity of G2-cells to chemicalsmight be due to shorter repair time of lesions induced in DNA, which are experessed as chromatid typeaberrations in the first mitosis.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Keyword(s): CEL CYCLE STAGE , MOLT-4 CELL LINE, CYTARABINE, METHOREXATE, CHROMOSOMAL ABERRATIONS
 
References: 
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