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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   1ST WEEK JANUARY 2013 , Volume 31 , Number 259; Page(s) 1769 To 1778.
 
Paper: 

EFFECT OF CHOLESTEROL, IRON AND VITAMIN E ON PROTAMINE DEFICIENCY AND DNA FRAGMENTATION OF MALE RABBIT SPERM

 
 
Author(s):  GHASEMI NAZEM, DASHTI GHOLAMREZA*, AMOOZGAR FATEMEH, VAEZ SEYED AHMAD
 
* DEPARTMENT OF ANATOMICAL SCIENCES AND MOLECULAR BIOLOGY, SCHOOL OF MEDICINE, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: The role of vitamin E as an antioxidant and iron-containing compounds on DNA is not well defined. So, recent study was carried out to determine the role of vitamin E and iron, as both separate and combined effect on sperm protamine deficiency and DNA fragmentation in male rabbits.
Methods: This study included 42 male rabbits which were randomly divided into seven groups each containing six rabbits. They were all treated under special diets for 6 weeks. After this period the animals were anesthetized and sacrificed. The epididymis duct and vas deferens were dissected and their sperms were extracted from it by Ham’s f10 solution. Sperm DNA fragmentation and protamine deficiency were evaluated by the acridine orange and chromomycin A3 staining, respectively.
Findings: The mean values of protamine deficiency and DNA fragmentation were significantly higher in group that concomitant use of cholesterol and iron. In addition, Vitamin E as an antioxidant agent has a significant effect in reducing these damages.
Conclusion: Vitamin E as an antioxidant, decrease sperm DNA damage resulting from the consumption of foods high in cholesterol and iron.

 
Keyword(s): CHOLESTEROL, IRON, VITAMIN E, DNA FRAGMENTATION, PROTAMINE DEFICIENCY, SPERM
 
 
References: 
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Citations: 
 
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APA: Copy

GHASEMI, N., & DASHTI, G., & AMOOZGAR, F., & VAEZ, S. (2013). EFFECT OF CHOLESTEROL, IRON AND VITAMIN E ON PROTAMINE DEFICIENCY AND DNA FRAGMENTATION OF MALE RABBIT SPERM. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 31(259), 1769-1778. https://www.sid.ir/en/journal/ViewPaper.aspx?id=400470



Vancouver: Copy

GHASEMI NAZEM, DASHTI GHOLAMREZA, AMOOZGAR FATEMEH, VAEZ SEYED AHMAD. EFFECT OF CHOLESTEROL, IRON AND VITAMIN E ON PROTAMINE DEFICIENCY AND DNA FRAGMENTATION OF MALE RABBIT SPERM. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S). 2013 [cited 2021September27];31(259):1769-1778. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=400470



IEEE: Copy

GHASEMI, N., DASHTI, G., AMOOZGAR, F., VAEZ, S., 2013. EFFECT OF CHOLESTEROL, IRON AND VITAMIN E ON PROTAMINE DEFICIENCY AND DNA FRAGMENTATION OF MALE RABBIT SPERM. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), [online] 31(259), pp.1769-1778. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=400470.



 
 
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