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Paper Information

Journal:   RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)   2003 , Volume 8 , Number 3 (33); Page(s) 207 To 211.
 
Paper: 

Antigen Retrieval Immunohistochemistry Under The Influence Of Ph

 
 
Author(s):  Karimi Sh.*, Azizi M.R.
 
* Department of Pathology, Shaheed Beheshti University of Medical Sciences
 
Abstract: 
Background: Antigen retrieval (AR) incorporate high temperature heating of tissue sections before immunostaining that strongly increases intensity of immunohistochemical staining.Materials and methods: We have tested the hypothesis that PH of the AR solution may influence the quality of immunostaining by using 3 different AR buffer solutions at 3 different PH values (2.7, 6.16 and 9.27). We have evaluated the staining of 9 antibodies on routinely formalin fixed embedded sections under different PH condition with microwave heating for 10 minutes. The intensity of immounostaining was graded by two pathologists.Results: Antigens have revealed different patterns of staining in different PH. Some antigens showed more or less equal intensity of immunohistochemical staining in these PH. Others showed a dramatic decrease in the intensity of AR immunohistochemical staining at PH=6.16. Still others showed their strongest intensity in PH=9.27.Conclusion: According to several studies concerning optimization of AR system and the aforementioned findings, standardization of AR system should therefore include optimization of the temperature and PH. Meanwhile, PH=9.27 seems to be suitable for most antigens to be effectively retrieved.
 
Keyword(s): ANTIGEN RETRIEVAL, IMMUNOHISTOCHEMICAL STAINING, PH
 
 
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APA: Copy

KARIMI, S., & AZIZI, M. (2003). ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY UNDER THE INFLUENCE OF PH. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 8(3 (33)), 207-211. https://www.sid.ir/en/journal/ViewPaper.aspx?id=3605



Vancouver: Copy

KARIMI SH., AZIZI M.R.. ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY UNDER THE INFLUENCE OF PH. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH). 2003 [cited 2022January24];8(3 (33)):207-211. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=3605



IEEE: Copy

KARIMI, S., AZIZI, M., 2003. ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY UNDER THE INFLUENCE OF PH. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), [online] 8(3 (33)), pp.207-211. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=3605.



 
 
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