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Paper Information

Journal:   MODARES JOURNAL OF MEDICAL SCIENCES (PATHOBIOLOGY)   SPRING 2013 , Volume 16 , Number 1; Page(s) 23 To 39.
 
Paper: 

DENDROSOMAL CURCUMIN INDUCED APOPTOSIS BY SUPPRESSION OF PLURIPOTENCY GENES IN 5637 BLADDER CANCER CELLS

 
 
Author(s):  TAHMASEBI MIRGANI MARYAM, SADEGHIZADEH MAJID*, NAJAFI FARHOOD, MOWLA SEYED JAVAD
 
* DEPARTMENT OF GENETICS, FACULTY OF BIOLOGICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Objective: The anti-cancer properties of curcumin, a poliphenol extract from the rhizome of curry, has been confirmed by many investigators. However, low levels of uptake, tissue distribution and rapid metabolism has limited its application as an anti-cancer drug. This study is aimed at increasing curcumin's water solubility due to a biodegradable, neutral and non-toxic micellar nano-carrier called dendrosome. This study intends to evaluate the role of dendrosomal-curcumin (DNC) in bladder cancer cell growth.
Methods: We performed the MTT assay, flow cytometry and Annexin V-FLUOS (as an apoptosis detection kit) to evaluate cell death. The genetic mechanism of DNC-induced apoptosis was accomplished by a study of the relative expressions of OCT4A, OCT4B1, SOX-2 and Nanog using real-time PCR.
Results: DNC-induced cell death complied with a time and dose-dependent paradigm in the 5637 cell line. Cell cycle analysis revealed that the number of cells increased in pre- G1 and gradually decreased in G1 and S phases. This showed the inhibitory property of dendrosomal-curcumin on DNA synthesis. Data from real-time PCR determined that expressions of OCT4A, OCT4B1, SOX-2 and Nanog could be related to 5637 cancer cell growth. Dendrosomal-curcumin significantly suppressed mRNA expression of the above mentioned genes (p<0.01).
Conclusion: The data showed that DNC induced apoptosis by suppression of pluripotency genes in 5637 bladder cancer cells, which confirmed the useful characteristic of nano-drug in bladder cancer therapy.

 
Keyword(s): CURCUMIN, DENDROSOME, CELL-PROGRAMMED DEATH, BLADDER CANCER, PLURIPOTENCY GENES
 
References: 
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