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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   2002 , Volume 31 , Number 3-4; Page(s) 133 To 137.
 
Paper: 

USE OF RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD-PCR) AND ITS2 PCR ASSAYS FOR DIFFERENTIATION OF POPULATIONS AND PUTATIVE SIBLING SPECIES OF ANOPHELES FLUVIATILIS (DIPTERA: CULICIDAE) IN IRAN

 
 
Author(s):  NADAF DEZFOULI S.R.*, OSHAGHI M.A., VATANDOUST H., JAVADIAN E., TELMADAREI Z., ASMAR M.
 
* DEPT. MEDICAL ENTOMOLOGY AND VECTOR CONTROL, SCHOOL OF PUBLIC HEALTH AND INSTITUTE OF HEALTH RESEARCH, TEHRAN UNIVERSITY OF MEDICAL SCIENCE, TEHRAN, IRAN
 
Abstract: 
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Anopheles fluviatilis complex is known to be a vector of malaria in Iran. Since mosquitoes of this species cover a wide geographical range in Iran, they might have evolved into different separated populations. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) assay was used to differentiate geographic populations of this species. DNA was extracted from individual mosquitoes from 8 localities in 4 south and southeast provinces and amplified in PCR reactions using 18 single primers of arbitrary nucleotide sequence. Results of RAPD-PCR showed that Kazeroun populations could simply be differentiated from other populations using a diagnostic fragment amplified with primer UBC-306. But other populations could not be differentiated either visually or by means of statistical analysis. Moreover ITS2 fragments of some selected specimens were amplified using a pair of universal primer and sequenced as a key standard for detection of putative sibling species. Sequence analysis of the ITS2 fragments revealed a very high (100%) homology among the populations. These findings are crucial in epidemiological studies concerning relatedness of geographic populations and vector movement in the region. Results of RAPD-PCR and ITS2 analysis suggest that this taxon in Iran comprises of only one species with a low genetic variation among geographic populations.

 
Keyword(s): ANOPHELES FLUVIATILIS, ITS2, RAPD-PCR, IRAN
 
References: 
 
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