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Paper Information

Journal:   IRANIAN JOURNAL OF BIOTECHNOLOGY   July 2004 , Volume 2 , Number 3; Page(s) 164 To 169.
 
Paper: 

DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES

 
 
Author(s):  NASIRI H., FOROUZANDEH M., RASAEI M.J., POURFATH ELAH A.A., RAHBARIZADEH F.
 
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Abstract: 
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We have developed an allotyping assay for detection of four HLA DRB1*01 group alleles based on polymerase chain reaction and solution hybridization in a microtiter plate. Using group specific primers a region within exon 2 of HLA DRB1 gene was amplified by PCR. Labeling of PCR product was achieved by adding small amount of Dig-dUTP in place of dTTP. Labeled PCR product was hybridized to allele (HLA DRB1*0101, *0102, *0103 and *0104) specific and a group (HLA DRB1*01) specific oligonucleotide probes in separate wells of the plate. Hybridized amplicones were detected by an enzymatic procedure. Ninety DNA samples were tested in parallel with PCR-SSP typing. The results were found to be well correlated by two methods. These results further suggest that, PCRELISA would be a rapid, specific and simple method that can be used for high resolution HLA typing before bone marrow and stem cell transplantation.
 
Keyword(s): HLA DRB1, PCR-ELISA, HYBRIDIZATION, GROUP SPECIFIC AMPLIFICATION
 
 
References: 
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Citations: 
 
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APA: Copy

NASIRI, H., & FOROUZANDEH, M., & RASAEI, M., & POURFATH ELAH, A., & RAHBARIZADEH, F. (2004). DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES . IRANIAN JOURNAL OF BIOTECHNOLOGY, 2(3), 164-169. https://www.sid.ir/en/journal/ViewPaper.aspx?id=32749



Vancouver: Copy

NASIRI H., FOROUZANDEH M., RASAEI M.J., POURFATH ELAH A.A., RAHBARIZADEH F.. DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES . IRANIAN JOURNAL OF BIOTECHNOLOGY. 2004 [cited 2021October23];2(3):164-169. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=32749



IEEE: Copy

NASIRI, H., FOROUZANDEH, M., RASAEI, M., POURFATH ELAH, A., RAHBARIZADEH, F., 2004. DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES . IRANIAN JOURNAL OF BIOTECHNOLOGY, [online] 2(3), pp.164-169. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=32749.



 
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