Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   BIOIMPACTS   2013 , Volume 3 , Number 2; Page(s) 101 To 104.
 
Paper: 

EFFECT OF HEMADO ON LEVEL OF CK-MB AND LDH ENZYMES AFTER ISCHEMIA/REPERFUSION INJURY IN ISOLATED RAT HEART

 
DOI: 

10.5681/bi.2013.003

 
Author(s):  AMANI MOHAMMAD, JEDDI SAJAD*, AHMADIASL NASSER, USEFZADE NASIBE, ZAMANI JALAL
 
* RESEARCH INSTITUTE FOR ENDOCRINE SCIENCES, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Introduction: Ischemia/Reperfusion (IR) injury mainly causes the increase of enzymes involved in myocytes injury including CK-MB (creatine kinase-MB) isoenzyme and LDH (lactate dehydrogenase). Leakage of CK-MB isoenzyme and LDH from myocardial tissues to blood is indicator of acute myocardial infarction. The aim of this study was to assess the effect of HEMADO on IR injury and its relationship with mitochondrial ATP-sensitive K+ channels (mitoKATP) in rat heart.
Methods: Twenty eight male Wistar rats (250-300g) were divided into four groups (seven members in each group): control (without ischemia), I/R (with ischemia+without HEMADO), ischemia received HEMADO (HEMADO), ischemia received HEMADO and 5-HD (5-hydroxydecanoate, specific mitoKATP channel blocker) (HEMADO+5-HD). The animals were anesthetized and the hearts were quickly removed and mounted on Langendorff apparatus and perfused by Krebs-Henseleit solution under constant pressure and temperature of 37oC. After 20 minutes of stabilization, ischemic groups were exposed to 40 minutes of global ischemia and consecutive 90 minutes of reperfusion.
Results: IR injury increased the level of LDH and CK-MB in the collected coronary flow during 5 minutes since start of reperfusion. HEMADO reduced the enzymes' levels and using 5-HD abolished the effect of HEMADO.
Conclusion: Our findings indicated that HEMADO could protect the heart against ischemia-reperfusion injury by decreasing the CK-MB and LDH levels. The cardioprotective effect of HEMADO may be mediated in part by mitoKATP.

 
Keyword(s): HEMADO, LACTATE DEHYDROGENASE, CREATINE KINASE, ISCHEMIA/REPERFUSION INJURY, MITOKATP
 
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
 
+ Click to Cite.
APA: Copy

AMANI, M., & JEDDI, S., & AHMADIASL, N., & USEFZADE, N., & ZAMANI, J. (2013). EFFECT OF HEMADO ON LEVEL OF CK-MB AND LDH ENZYMES AFTER ISCHEMIA/REPERFUSION INJURY IN ISOLATED RAT HEART. BIOIMPACTS, 3(2), 101-104. https://www.sid.ir/en/journal/ViewPaper.aspx?id=319456



Vancouver: Copy

AMANI MOHAMMAD, JEDDI SAJAD, AHMADIASL NASSER, USEFZADE NASIBE, ZAMANI JALAL. EFFECT OF HEMADO ON LEVEL OF CK-MB AND LDH ENZYMES AFTER ISCHEMIA/REPERFUSION INJURY IN ISOLATED RAT HEART. BIOIMPACTS. 2013 [cited 2021November27];3(2):101-104. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=319456



IEEE: Copy

AMANI, M., JEDDI, S., AHMADIASL, N., USEFZADE, N., ZAMANI, J., 2013. EFFECT OF HEMADO ON LEVEL OF CK-MB AND LDH ENZYMES AFTER ISCHEMIA/REPERFUSION INJURY IN ISOLATED RAT HEART. BIOIMPACTS, [online] 3(2), pp.101-104. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=319456.



 
  pdf-File
Yearly Visit 333
 
 
Latest on Blog
Enter SID Blog