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Paper Information

Journal:   SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES JOURNAL   APRIL-MAY 2013 , Volume 15 , Number 1; Page(s) 1 To 10.
 
Paper: 

THE SYNERGIC EFFECTS OF SAFFRON AQUA EXTRACT AND LOW FREQUENCY ELECTROMAGNETIC FIELD ON ANGIOGENESIS IN CHICK CHORIOALANTOIC MEMBRANE

 
 
Author(s):  MOUSAVI M., BAHARARA J.*, ZAFAR BALANEZHAD S., NEJAD SHAHROKHABADI KH.
 
* BIOLOGY DEPT., MASHHAD BRANCH, ISLAMIC AZAD UNIVERSITY, MASHHAD, I.R. IRAN
 
Abstract: 

Background and aims: Studies both in vivo and in vitro shows anti-cancer effects of saffron.
Angiogenesis is required for many physiological and pathological conditions such as tumor growth. On the other hand, many processes are affected by electromagnetic fields. In this study the synergic effects of saffron aqua extract and low-frequency electromagnetic field on angiogenesis were investigated.
Methods: In this laboratory experimental study, ross fertilized eggs were divided in 9 groups, including: control, first and second sham-exposed groups.1, 2 and 3 experimental groups (treated by the saffron extract concentrations, 100, 400 and 800
mg/ml) and experimental groups, 4, 5 and 6 (synergic treatment with the saffron with same concentrations which exposed on 400 gauss intensity electromagnetic field). In the second day of incubation, the window was open on eggs in sterile conditions on the eighth day, 10 ml of Saffron extract was injected. In the tenth day in sham- exposed 2 groups and 4, 5 and 6 experimental groups eggs exposed on electromagnetic field for 4 hours. At last in the twelfth day all cases were photographed by photo stereomicroscope and both numbers and lengths of vessels around the sponges were measured by Image J software, data were analyzed with SPSS and by statistic test such as tukey & ANOVA.
Result: Data analysis, shows that average length of blood vessels in the experimental group 1, compared with the control group showed no significant decrease (P>0.05), while in experimental group 2 and 3 it was significantly increased (P<0.001). The average number of blood vessels in the experimental group 1, 2 and 3 decline noticeably in compare with the control group (P<0.05). Comparing of branches in blood vessels in second sham-exposed with the control group shows dramatically reduction (P<0.001). Analysis the data reveals that the number of blood vessels and length of branches in the experimental 4 group significantly decreased, P<0.05, while this reduction in the fifth and sixth one were significant (P<0.001).
Conclusion: The aqueous extract of saffron has dose dependently inhibitory effects on angiogenesis which influenced by low-frequency electromagnetic field with 400 Gauss intensity significantly.

 
Keyword(s): AANGIOGENESIS, CHORIOALANTOIC CHICKEN MEMBRANE, CANCER, ELECTROMAGNETIC FIELD, SAFFRON
 
References: 
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