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Paper Information

Journal:   IRANIAN JOURNAL OF MICROBIOLOGY   SEPTEMBER 2012 , Volume 4 , Number 3; Page(s) 118 To 123.
 
Paper: 

MOLECULAR IDENTIFICATION AND DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSAISOLATED FROM DIFFERENT INFECTIOUS ORIGINS

 
 
Author(s):  NIKBIN V.S., ASLANI MOHAMMAD MEHDI*, SHARAFI Z., HASHEMI POUR M., SHAH CHERAGHI F., EBRAHIMIPOUR GH.
 
* DEPARTMENT OF MICROBIOLOGY, PASTEUR INSTITUTE OF IRAN, PASTEUR STREET, TEHRAN, IRAN
 
Abstract: 

Background and Objectives: Pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity. The aim of this study was to evaluate opr I, oprL and toxA genes for PCR identification of clinical P. aeruginosa. In order to find out any relation between special virulence factors and special manifestation of P. aeruginosa infections, we detected virulence factors among these isolates by PCR. Ribotyping was used to evaluate the clonal relationship between strains with the same genetic patterns of the genes studied.
Materials and Methods: In this study, 268 isolates of P. aeruginosa were recovered from burn, wound and pulmonary tract infections. The prevalence of oprI, oprL, toxA, lasB, exoS and nan1 genes was determined by PCR. One hundred and four isolates were selected randomly to investigate clonal diversity of the isolates with ribotyping using Sma I.
Results and Conclusions: All P. aeruginosa isolates in this study carried oprI, oprL and lasB genes. Difference between exo Sprevalence in isolates from pulmonary tract and burn isolates was statistically significant. Prevalence of nan1 and toxA gene was significantly higher in pulmonary tract and burn isolates, respectively. Ribotyping showed that most of the isolates (87%) belonged to clone A and B. Detection of oprI, oprL and tox A genes by PCR is recommended for molecular identification of P. aeruginosa. Determination of different virulence genes of P. aeruginosa isolates suggests that they are associated with different levels of intrinsic virulence and pathogenicity. Ribotyping showed that strains with the same genetic patterns of the genes do not necessarily have similar ribotype patterns.

 
Keyword(s): PSEUDOMONAS AERUGINOSA, RIBOTYPING, VIRULENCE FACTORS
 
 
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Citations: 
 
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APA: Copy

NIKBIN, V., & ASLANI, M., & SHARAFI, Z., & HASHEMI POUR, M., & SHAH CHERAGHI, F., & EBRAHIMIPOUR, G. (2012). MOLECULAR IDENTIFICATION AND DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSAISOLATED FROM DIFFERENT INFECTIOUS ORIGINS. IRANIAN JOURNAL OF MICROBIOLOGY, 4(3), 118-123. https://www.sid.ir/en/journal/ViewPaper.aspx?id=318392



Vancouver: Copy

NIKBIN V.S., ASLANI MOHAMMAD MEHDI, SHARAFI Z., HASHEMI POUR M., SHAH CHERAGHI F., EBRAHIMIPOUR GH.. MOLECULAR IDENTIFICATION AND DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSAISOLATED FROM DIFFERENT INFECTIOUS ORIGINS. IRANIAN JOURNAL OF MICROBIOLOGY. 2012 [cited 2021October29];4(3):118-123. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=318392



IEEE: Copy

NIKBIN, V., ASLANI, M., SHARAFI, Z., HASHEMI POUR, M., SHAH CHERAGHI, F., EBRAHIMIPOUR, G., 2012. MOLECULAR IDENTIFICATION AND DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSAISOLATED FROM DIFFERENT INFECTIOUS ORIGINS. IRANIAN JOURNAL OF MICROBIOLOGY, [online] 4(3), pp.118-123. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=318392.



 
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