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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   FALL 2013 , Volume 15 , Number 3 (59); Page(s) 218 To 223.
 
Paper: 

COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS

 
 
Author(s):  DELIREZH NOWRUZ*, SHOJAEEFAR EHSAN, PARVIN PARVA, ASADI BEHNAZ
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF VETERINARY MEDICINE, URMIA UNIVERSITY, URMIA, IRAN
 
Abstract: 
Objective: It is believed that monocyte isolation methods and maturation factors affect the phenotypic and functional characteristics of resultant dendritic cells (DC). In the present study, we compared two monocyte isolation methods, including plastic adherence-dendritic cells (Adh-DC) and magnetic activated cell sorting- dendritic cells (MACS-DC), and their effects on phagocytic activity of differentiated immature DCs (imm DCs).
Materials and Methods: In this experimental study, imm DCs were generated from plastic adherence and MACS isolated monocytes in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) in five days. The phagocytic activity of imm DCs was analyzed by fluorescein isothiocyanate (FITC)-conjugated latex bead using flow cytometry. One way ANOVA test was used for statistical analysis of differences among experimental groups, including Adh-DC and MACS-DC groups.
Results: We found that phagocytic activity of Adh-DC was higher than MACS-DC, whereas the mean fluorescence intensity (MFI) of phagocytic cells was higher in MACS-DC (p<0.05).
Conclusion: We concluded that it would be important to consider phagocytosis parameters of generated DCs before making any decision about monocyte isolation methods to have fully functional DCs.
 
Keyword(s): MONOCYTE, DENDRITIC CELLS, PHAGOCYTOSIS, MACS, ADHERENCE
 
 
References: 
 
Citations: 
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APA: Copy

DELIREZH, N., & SHOJAEEFAR, E., & PARVIN, P., & ASADI, B. (2013). COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS. CELL JOURNAL (YAKHTEH), 15(3 (59)), 218-223. https://www.sid.ir/en/journal/ViewPaper.aspx?id=316838



Vancouver: Copy

DELIREZH NOWRUZ, SHOJAEEFAR EHSAN, PARVIN PARVA, ASADI BEHNAZ. COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS. CELL JOURNAL (YAKHTEH). 2013 [cited 2021August03];15(3 (59)):218-223. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=316838



IEEE: Copy

DELIREZH, N., SHOJAEEFAR, E., PARVIN, P., ASADI, B., 2013. COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS. CELL JOURNAL (YAKHTEH), [online] 15(3 (59)), pp.218-223. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=316838.



 
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