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Paper Information

Journal:   IRANIAN JOURNAL OF BIOLOGY   Fall-Winter 2002-2003 , Volume 13 , Number 3-4; Page(s) 39 To 48.
 
Paper: 

SOMATIC EMBRYOGENESIS FROM EXPLANTS OF SOME SPECIES OF HYOSCYAMUS: H. PUSILLUS L., H. ARECHNOIDOUS POJARK AND H. NIGER L.

 
 
Author(s):  EBRAHIMZADEH M., MAJD A.
 
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Abstract: 
With the culture of various explant under certain cultural conditions, embryogenesis will result. In this research mbryos were obtained from several species of Hyoscyamus: H.arachnoideus H.pusillus, H.niger, from 2 distinct geographical regions, on MS mediums with various hormonal treatments. The results from statistical assessments showed that the highest embryogenesis were obtained with the following conditions: Culture of region 2 biennial H.niger stern segments on mediums of No .I (IAA=0.2mg/L), No. 3 (BAP=1.5mg/L), No. 4 (Kin=1.5mg/L), No. 7 (BAP=1.5, IAA=0.2mg/L), and No. 10 (Kin= 1.5, IAA=0.2mg/L). Culture of region 2 biennial H. niger roots on mediums of No. 4, 7, 10, and (BAP=1.12, IAA=0.09mg/L). Culture of region 2 biennial H. niger leaves on medium contianing Kin (1.5 mg/L). In addition, the highest embryogenesis frequencies were observed with: Culture of H.pusillus sterns on mediums No. 7; and 12. Culture of H.pusillus leaves on medium No. 3. Culture of H.arachnoideus leaves and sterns on mediums No. 7, and 12. Culture of region I biennial H.niger sterns on mediums No. 7, and 12. Culture of region 2 biennial H.niger roots, sterns, and leaves on medium No. 4, 7, and 12. Culture of region 2 biennial Hniger roots, and sterns on mediums No. 1, 3, and 10. The formation of plantlet resulted from the subculture of the obtained embryos on MS medium and MS medium contianing IAA (0.2mg/L).
 
Keyword(s): EMBRYOGENESIS, HYOSCYAMUS SPECIES, IN VITRO CULTURE
 
 
References: 
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Click to Cite.
APA: Copy

EBRAHIMZADEH, M., & MAJD, A. (2003). SOMATIC EMBRYOGENESIS FROM EXPLANTS OF SOME SPECIES OF HYOSCYAMUS: H. PUSILLUS L., H. ARECHNOIDOUS POJARK AND H. NIGER L. . IRANIAN JOURNAL OF BIOLOGY, 13(3-4), 39-48. https://www.sid.ir/en/journal/ViewPaper.aspx?id=30314



Vancouver: Copy

EBRAHIMZADEH M., MAJD A.. SOMATIC EMBRYOGENESIS FROM EXPLANTS OF SOME SPECIES OF HYOSCYAMUS: H. PUSILLUS L., H. ARECHNOIDOUS POJARK AND H. NIGER L. . IRANIAN JOURNAL OF BIOLOGY. 2003 [cited 2021May08];13(3-4):39-48. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=30314



IEEE: Copy

EBRAHIMZADEH, M., MAJD, A., 2003. SOMATIC EMBRYOGENESIS FROM EXPLANTS OF SOME SPECIES OF HYOSCYAMUS: H. PUSILLUS L., H. ARECHNOIDOUS POJARK AND H. NIGER L. . IRANIAN JOURNAL OF BIOLOGY, [online] 13(3-4), pp.39-48. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=30314.



 
 
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