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Paper Information

Journal:   JOURNAL OF CELL & TISSUE   FALL 2012 , Volume 3 , Number 3; Page(s) 211 To 220.
 
Paper: 

NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS

 
 
Author(s):  AKBARI S., PARVANEH TAFRESHI A.*, ABBASI SH., MOMEN H., MASOUMI M.
 
* DEPARTMENT OF BASIC SCIENCES IN BIOTECHNOLOGY, THE NATIONAL RESEARCH INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY, TEHRAN, IRAN
 
Abstract: 

Aim: In this study, gene transfer efficiency of human lentiviral vector (HIV) into rat bone marrow stromal cells was compared with that of the feline lentiviral vector (FIV).
Material and Methods: To produce lentiviral vectors, a triple transfection with a shuttle vector, a backbone which carries either the GFP gene as a control or the NGF gene, and an envelope vector was performed using calcium-phosphate method in HEK-293T cell line. Supernatants of HEK-293T cell line containing the produced viruses were then exposed to bone marrow stromal cells (BMSCs) of the rat.
Results: While bone marrow stromal cells infected with HIV-NGF showed a relatively high immunoreactivity of NGF, those infected with FIV-NGF did not express NGF. Furthermore, BMSCs infected with FIV-NGF viruses containing antibiotic resistant gene could not survive in the culture media containing the antibiotic.
Conclusion: FIV based lentiviral vectors may not have a desirable efficacy for gene transfer into bone marrow stromal cells.

 
Keyword(s): FELINE IMMUNODEFICIENCY VIRUS LENTIVIRAL VECTOR, HUMAN IMMUNODEFICIENCY VIRUS LENTIVIRAL VECTOR, BONE MARROW STROMAL CELLS, NGF
 
 
References: 
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Cite:
APA: Copy

AKBARI, S., & PARVANEH TAFRESHI, A., & ABBASI, S., & MOMEN, H., & MASOUMI, M. (2012). NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS. JOURNAL OF CELL & TISSUE, 3(3), 211-220. https://www.sid.ir/en/journal/ViewPaper.aspx?id=297765



Vancouver: Copy

AKBARI S., PARVANEH TAFRESHI A., ABBASI SH., MOMEN H., MASOUMI M.. NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS. JOURNAL OF CELL & TISSUE. 2012 [cited 2021April19];3(3):211-220. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=297765



IEEE: Copy

AKBARI, S., PARVANEH TAFRESHI, A., ABBASI, S., MOMEN, H., MASOUMI, M., 2012. NGF GENE TRANSFER TO BONE MARROW MESENCHYMAL STROMAL CELLS USING FIV AND HIV BASED LENTIVIRAL VECTORS. JOURNAL OF CELL & TISSUE, [online] 3(3), pp.211-220. Available: <https://www.sid.ir/en/journal/ViewPaper.aspx?id=297765>.



 
 
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