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Paper Information

Journal:   JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES   SUMMER 2012 , Volume 19 , Number 2 (51); Page(s) 148 To 156.
 
Paper: 

SYNERGIC EFFECTS OF ATORVASTATIN AND LOW FREQUENCY ELECTROMAGNETIC FIELD ON CHORIOALLANTOIC MEMBRANE ANGIOGENESIS OF CHICK EMBRYO

 
 
Author(s):  BAHARARA JAVAD*, ZAFAR BALANEZHAD SAEIDEH, NEJAD SHAHROKH ABADI KHADIJEH, HESAMI ZAHRA
 
* DEPT. OF BIOLOGY, MASHHAD BRANCH, ISLAMIC AZAD UNIVERSITY, MASHHAD, IRAN
 
Abstract: 

Background and Aim: Statins promote the proliferation and survival of endothelial cells. Electromagnetic field, too, affects angiogenesis. In the present research, the effect of atorvastatin together with a low frequency electromagnetic field (50 Gauss) on angiogenesis of chick embryo was assessed.
Materials and Methods: 98 fertilized eggs were divided into 7 equal groups in the following manner: control group and experimental ones 1) lab case, 2) atorvastatin (0.1
mm) group, 3) atorvastatin (10 mm) group, 4) electromagnetic field group, 5) atorvastatin (0.1 mm) and electromagnetic field group, 6) atorvastatin (10 mm) and electromagnetic field group. On the eighth day of incubation a gelatin sponge was placed on chorioallantoic membrane (CAM) and was soaked with 10 microliters of atorvastatin (0/1 mm) for groups 2, 5, 3, and 6); and for groups 3 and 6 ten microliter of atorvastatin (10 mm) On the 10th day the eggs of the groups 4, 5, and 6 were put under an electromagnetic field for four hours. On the twelfth day, incubation of angiogenesis and respective length of vessels in all samples was measured. The obtained data was statistically analyzed by means of SPSS software (v.16), ANOVA and Tukey test P<0.05.
Results: Mean number and length of vessels in the control and experimental cases didn’t show any significant differences (p>0.05). Comparison between mean number and length of vessels in the control and group 2 showed a significant increase (p=0.001), but groups 3 and 6 was showed a significant decrease in the mean number and length of vessels compared with the controls (p<0.001). Comparison between mean length of vessels in the control and group 4 revealed a significant decrease in the average length of vessels (p=0.001).
Conclusion: Synergistic effects of atorvastatin (10
mm) and electromagnetic field cause inhibition of angiogenesis in chorioallantoic membrane. Besides, electromagnetic field has an inhibitory effect on stimulatory effect of atorvastatin 0.1 mm on angiogenesis.

 
Keyword(s): ANGIOGENESIS, ATORVASTATIN, ELECTROMAGNETIC FIELD
 
References: 
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