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Paper Information

Journal:   POLICE MEDICINE   SUMMER 2012 , Volume 1 , Number 1 (1); Page(s) 28 To 35.
 
Paper: 

EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE

 
 
Author(s):  SALEHI CHALESHTORI A.R., SAADATI M.*, AHSANI ARANI Y., BAHADOR A., HOSSEINI M., HESARAKI M., DOROUDIAN M.
 
* FACULTY OF BASIC SCIENCES, DEPARTMENT OF MOLECULAR CELL BIOLOGY, IMAM HUSSEIN UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Aim: This study aims to detect the ipaD plasmid in Shigella dysenteriae type 1 and evaluate the invasive ability of a live, attenuatedShigella dysenteriae type 1 generated from deletion of theipaD plasmid. The attenuated strain was tested in guinea pigs.
Materials and Methods: We performed biochemical and antibody tests in addition to PCR analysis, to identify the strain and serotype of an isolatedShigella strain. The plasmid version of theipaD gene was recognized. We generated a live, attenuated Shigellaserotype by deletion of the plasmid version of the ipaD gene, which was confirmed by the above mentioned tests. Absence of theipaD gene was assessed in a PCR reaction and compared to the wild type strain. Various dilutions of cultured bacteria were prepared and colony numbers counted. We prepared specific dilution which was appropriate for conducting the keratoconjunctive test. We used 6 guinea pigs that were divided into two groups, control (n=3) and experimental (n=3). C Invasion capability of produced strain was evaluated by keratoconjunctive test in guinea pigs.
Results: The produced live attenuated Shigella dysenteriae type 1 was confirmed through serological tests and PCR. Absence ofipaD was confirmed. Results of keratoconjunctive test has shown that this strain lost its ability to invade host cells.
Conclusion: The plasmid version of ipaD gene plays a critical role in Shigella invasiveness. Deletion of mentioned gene reduces the invasion ability of these bacteria. Therefore, Live attenuated
DipaD shigella dysenteriae can be an appropriate candidate to produce vaccine against shigellosis.

 
Keyword(s): SHIGELLA DYSENTERIAE TYPE 1, LIVE ATTENUATED, KERATOCONJUNCTIVE, GUINEA PIG, VACCINE CANDIDATE
 
 
References: 
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Citations: 
 
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APA: Copy

SALEHI CHALESHTORI, A., & SAADATI, M., & AHSANI ARANI, Y., & BAHADOR, A., & HOSSEINI, M., & HESARAKI, M., & DOROUDIAN, M. (2012). EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE. POLICE MEDICINE, 1(1 (1)), 28-35. https://www.sid.ir/en/journal/ViewPaper.aspx?id=282331



Vancouver: Copy

SALEHI CHALESHTORI A.R., SAADATI M., AHSANI ARANI Y., BAHADOR A., HOSSEINI M., HESARAKI M., DOROUDIAN M.. EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE. POLICE MEDICINE. 2012 [cited 2021October23];1(1 (1)):28-35. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=282331



IEEE: Copy

SALEHI CHALESHTORI, A., SAADATI, M., AHSANI ARANI, Y., BAHADOR, A., HOSSEINI, M., HESARAKI, M., DOROUDIAN, M., 2012. EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE. POLICE MEDICINE, [online] 1(1 (1)), pp.28-35. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=282331.



 
 
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