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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   SPRING 2007 , Volume 2 , Number 1; Page(s) 41 To 46.
 
Paper: 

ISOLATION OF ASTAXANTHIN OVERPRODUCING MUTANT OF PHAFFIA RHODOZYMA JH-82

 
 
Author(s):  AHMADIAN A.R., GOLKHOU SH., AMIN P., AZIZ SOLTANI M., BARAN TALAB F., DELARAM M.
 
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Abstract: 

Astaxanthin is a xanthophylls, a fat-soluble, oxygenated pigment a member of the carotenoid family. It has a unique molecular structure that 500 times-gives it powerful antioxidant function. Astaxanthin has 100 the antioxidant capacity of Vitamin E and 10 times the antioxidant capacity of beta-carotene and also stronger antioxidant than lutein, lycopene and tocotrienols. Mutagenesis of Phaffia rhodozyma was done with different concentrations of NTG. After many times of selections, mutated colonies were obtained that were resistant to 0.05% 2-Deoxyglucose. These were tested in GPY broth for astaxanthin formation. Four mutant’s colonies obtained with 250mg/ml of NTG. The highly pigmented mutants produced approximately 485 mg of total carotenoid per g yeast estimated as astraxanthin, compared with the parental strain which had 250 mg/g. The results of TLC analysis of carotenoid composition of P. rhodozyma mutant indicated that mutant strain produced five different carotenoids on the basis of polarity and RF values.

 
Keyword(s): PHAFFIA, HEMMATOCCOCUS, ASTAXANTHIN
 
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