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Paper Information

Journal:   JOURNAL OF MEDICINAL PLANTS   MARCH 2012 , Volume 11 , Number SUPPLEMENT 8; Page(s) 208 To 217.
 
Paper: 

DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION (MIC) OF ZATARIA MULTIFLORA BOISS. ESSENTIAL OIL AND LYSOZIM ON E. COLI O157: H7

 
 
Author(s):  HOSSEINZADEH A., MOHAJERFAR T., AKHONDZADEH BASTI A.*, KHANJARI A., GANDOMI NASRABADI H., MISAGHI A., SADEGHI S.
 
* DEPARTMENT OF FOOD HYGIENE, FACULTY OF VETERINARY MEDICINE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Background: In order to reduce or eliminate foodborne pathogens or food spoilage agents many research have been done to find natural preservatives.
Objective: This study was carried out to determine minimum inhibitory concentration (MIC) of lysozyme and zataria multiflora Boiss. Essential oil on, E. coli O157: H7 and their sub inhibitory concentration effect on bacterial growth curve.
Methods: In this study different concentrations of lysozyme (0, 125, 250, 500, 1000 μg/ml) and Zatria multiflora Boiss. Essential oil (0, 0.005, 0.01, 0.04, 0.08%) were used alone and in combination on BHI broth to determine MIC of zataria multiflora Boiss.essential oil and lysozyme with macro dilution and micro dilution methods and effect of sub inhibitory concentrations of them on bacterial growth curve of E. coil O157:H7.
Results: MIC of essential oil in two methods was achieved ٪0.04 whereas Lysozyme in (1000 µg/ml) concentration was not able to reduce bacterial growth. Furthermore combination results revealed that high concentration of lysozyme was not able to reduce calculated amount of MIC of essential oil. Sub inhibitory concentration effect of EO and lysozyme showed that their combination result in the increase of lag phase and reduction of bacterial growth.
Conclusion: utilization of EO and lysozyme together did not decrease MIC but combination of them increase lag phase.

 
Keyword(s): ZATARIA MULTIFLORA, LYSOZYME, MINIMUM INHIBITORY CONCENTRATION, E. COLI O157: H7
 
References: 
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