Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)   SPRING 2011 , Volume 14 , Number 1; Page(s) 81 To 88.
 
Paper: 

STUDY OF ANTIBIOTIC SUSCEPTIBILITY PATTERN AND PRESENCE OF IPAH GENE AMONG SHIGELLA STRAINS ISOLATED FROM SELECTED PROVINCES IN IRAN

 
 
Author(s):  GHANDIAN SHADI, SATTARI MORTEZA, NIKBIN VAJIHEH SADAT, ASLANI MOHAMMAD MEHDI
 
* 
 
Abstract: 

Objective: Shigellosis is one of the most common causes of morbidity and mortality in children with diarrhea in developing countries. It is essential to assess the antibiotic resistance patterns of these bacteria. ipaH gene is one of the virulence factors which can be used for detection of Shigella spp.
Materials and Methods: Total of 100 isolates of Shigella were collected from different provinces of Iran. This isolates were characterized by biochemical tests and serological tests using polyclonal antisera for 4 species of S. dysenteriae, S. sonnei, S. boydii and S. flexneri. Antibiotic susceptibility assay for 14 different antibiotics was carried out using agar disc diffusion method. Presence of ipaH gene was investigated by PCR using specific primers.
Results: From the results of this study the Shigella isolates were classified as follows: 36 (%73) Shigella sonnei, 9 (%18) Shigella flexneri, 3 (%5) Shigella boydii, 2 (%4) Shigella dysenteriae. Approximately %50 of the Shigella isolates were resistant to Tetracycline and Cotrimoxazole. Shigella sonnei showed more resistance than other serotypes against the studied antibiotics. PCR assays showed that all isolates harbored ipaH gene.
Conclusion: The results showed that prevalence of Shigella sonnei is higher than other serotypes. The isolates showed high sensitivity to third generation cephalosporines and aminoglycosides. PCR detection of ipaH gene as a reliable marker for identification of Shigella species could be recommended.

 
Keyword(s): SHIGELLA, ANTIBIOTIC SENSITIVITY, IPAH GENE, IRAN
 
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
 
+ Click to Cite.
APA: Copy

GHANDIAN, S., & SATTARI, M., & NIKBIN, V., & ASLANI, M. (2011). STUDY OF ANTIBIOTIC SUSCEPTIBILITY PATTERN AND PRESENCE OF IPAH GENE AMONG SHIGELLA STRAINS ISOLATED FROM SELECTED PROVINCES IN IRAN. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 14(1), 81-88. https://www.sid.ir/en/journal/ViewPaper.aspx?id=234180



Vancouver: Copy

GHANDIAN SHADI, SATTARI MORTEZA, NIKBIN VAJIHEH SADAT, ASLANI MOHAMMAD MEHDI. STUDY OF ANTIBIOTIC SUSCEPTIBILITY PATTERN AND PRESENCE OF IPAH GENE AMONG SHIGELLA STRAINS ISOLATED FROM SELECTED PROVINCES IN IRAN. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES). 2011 [cited 2021October20];14(1):81-88. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=234180



IEEE: Copy

GHANDIAN, S., SATTARI, M., NIKBIN, V., ASLANI, M., 2011. STUDY OF ANTIBIOTIC SUSCEPTIBILITY PATTERN AND PRESENCE OF IPAH GENE AMONG SHIGELLA STRAINS ISOLATED FROM SELECTED PROVINCES IN IRAN. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), [online] 14(1), pp.81-88. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=234180.



 
 
Persian Abstract Yearly Visit 67
 
 
Latest on Blog
Enter SID Blog