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Paper Information

Journal:   IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)   2010 , Volume 41 , Number 4; Page(s) 803 To 810.
 
Paper: 

ANALYSIS OF CAMV35S PROMOTER ACTIVITY IN DIFFERENT TISSUES OF TRANSGENIC TOBACCO PLANTS

 
 
Author(s):  ABBASI A., ABOOEI MEHRIZI F.*, FOOL L., SONOALL O.
 
* UNIVERSITY COLLEGE OF AGRICULTURE & NATURAL RESOURCES, UNIVERSITY OF TEHRAN
 
Abstract: 
Each gene consists of three major parts namely promoter, the main body of the gene and terminator. Without the promoter, there whould be no gene expression. In general, promoter is specific for each gene, and leads to the regulation of the final product of the gene. One of the most common promoters employed by plant genetic engineer is CaMV35S. Although it is assumed that CaMV35S is a constitutive promoter, but some reports suggest that this promoter is not expressed in all tissues and cell types. In addition, full accessible information regarding the expression profile of this promoter in all cells, in different tissues, and at the initial stages of development is lacking.
Considering the above mentioned limitations, it leads one to study in more detail the CaMV35Spromoter activity. For an analysis of the promoter activity, tobacco transgenic plants in which one of the interfering committed steps in their vitamin E biosynthesis had been inhibited through RNAi system were employed. Silencing of the gene involved in this interfering step resulted in reduced content of vitamin E in the transgenic plant as compared with the wild type. Since as a whole, vitamin E content will undergo reduction in transgenic plants, the vitamin’s content in such different tissues as leaves, root, flower organs, immature and mature seeds of tobacco transgenic vs. wild type plants was measured. The data recorded here demonstrate thatCaMV35S promoter has highly expressed activity in leaves and flower organ, moderate expression activity in roots and seeds, and while very lowly expressed in mature seeds.
 
Keyword(s): CAMV35S PROMOTER, CONSTITUTIVE PROMOTER, RNAI, MATURE SEED, TOBACCO PLANT, TRANSGENIC PLANT, GENE EXPRESSION
 
References: 
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