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Paper Information

Journal:   SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL   WINTER 2011 , Volume 6 , Number 4 (29); Page(s) 0 To 0.
 
Paper: 

EFFECTS OF GNRH TREATMENT ON DAY 6 OF THE ESTROUS CYCLE ON PROGESTERONE AND ESTRADIOL PATTERNS IN HOLSTEIN CATTLE

 
 
Author(s):  ARIANEJAD M., KOHRAM H., TOWHIDI A.
 
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Abstract: 

In this study, effects of GnRH treatment on the pattern of the estradiol and progesterone (P4) concentrations were studied during estrous cycle of Holstein cattle. Ten dairy cows were synchronized with two PGF2a injection which given 11 days apart. Cows were assigned randomly in control and GnRH groups. GnRH administered on day 6 of the estrous cycle in GnRH group. Haparinized blood samples were collected once daily from the caudal vein through the estrous cycle. Hormones were measured using Radioimmunoassay (Human Kit). Mixed procedure of SAS was used for data analyzing. Results showed that progesterone concentrations were not significantly different between two groups during estrous cycle. Progesterone concentration was less than 1 ng/ml between days 1-4 ± 2, then increased to maximum concentration on days 12 ± 3 of the estrous cycle. Thereafter, it decreased in day 15 until day 19, however it was still higher than 1 ng/ml. After day 19 of the estrous cycle, P4 concentration decreased to less than 1 ng/ml. Estradiol concentrations were not also significantly different between two groups during estrous cycle. In both groups Estradiol concentrations had the variations from 1.5±0.8 to 6.0±2.1 without any significant differences from days 1 to days 15±2. Thereafter, it increased from day 17 till end of estrous cycle and reached the maximum level on days 22±2 of the estrous cycle. The pattern of estradiol and progesterone concentrations was not significantly different between control and GnRH treatment animals during estrous cycle.

 
Keyword(s): COW, ESTROUS CYCLE, GNRH, PROGESTERONE, ESTRADIOL
 
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