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Paper Information

Journal:   JOURNAL OF CHEMICAL AND PETROLEUM ENGINEERING (JOURNAL OF FACULTY OF ENGINEERING)   MARCH 2010 , Volume 43 , Number 2; Page(s) 33 To 39.
 
Paper: 

IMMOBILIZATION OF ALKALINE PROTEASE PRODUCED FROM BACILLUS LICHENIFORMIS PTCC1331 ON CALCIUM ALGINATE (ITS EFFECT ON ENZYMATIC ACTIVITY)

 
 
Author(s):  SAFARIYOUN POUR SH., YAGHMAEI S.*, GHOBADINEZHAD Z.
 
* CHEMICAL AND PETROLEUM ENGINEERING DEPARTMENT, SHARIF UNIVERSITY OF TECHNOLOGY, I.R. IRAN
 
Abstract: 

Alkaline Protease enzyme produced from Bacillus Licheniformis PTCC1331 in optimum Condition(Temperature 37°C, 150 rpm, PH=10), is immobilized in different concentrations of Sodium Alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. Maximum enzymatic activity of 46[u/g]carrier was obtained in 2%w/v Sodium alginate solution. Investigations on different temperatures on enzymatic activity of free and immobilized enzymes shows that relative activity of free enzyme decreases in temperatures more than 45°C but immobilized enzyme maintains its activity up to 55°C and is more stable than free enzyme. Moreover stability of free and immobilized enzyme is studied in 40 & 50°C. Immobilized enzyme is more stable in 40°C than 50°C. Effect of pH on free and immobilized enzyme is also studied between pH 7 to 12.Both free and immobilized enzymes are more stable in pH 7 to 8 and activity of immobilized enzyme is more than free enzyme in this range. As it is resulted using the best method of immobilization, enzyme would be more stable and its usage would be more effective in industry.

 
Keyword(s): BACILLUS LICHENIFORMIS, ALKALINE PROTEASE, IMMOBILIZATION, CALCIUM ALGINATE
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

SAFARIYOUN POUR, S., & YAGHMAEI, S., & GHOBADINEZHAD, Z. (2010). IMMOBILIZATION OF ALKALINE PROTEASE PRODUCED FROM BACILLUS LICHENIFORMIS PTCC1331 ON CALCIUM ALGINATE (ITS EFFECT ON ENZYMATIC ACTIVITY). JOURNAL OF CHEMICAL AND PETROLEUM ENGINEERING (JOURNAL OF FACULTY OF ENGINEERING), 43(2), 33-39. https://www.sid.ir/en/journal/ViewPaper.aspx?id=182487



Vancouver: Copy

SAFARIYOUN POUR SH., YAGHMAEI S., GHOBADINEZHAD Z.. IMMOBILIZATION OF ALKALINE PROTEASE PRODUCED FROM BACILLUS LICHENIFORMIS PTCC1331 ON CALCIUM ALGINATE (ITS EFFECT ON ENZYMATIC ACTIVITY). JOURNAL OF CHEMICAL AND PETROLEUM ENGINEERING (JOURNAL OF FACULTY OF ENGINEERING). 2010 [cited 2021October19];43(2):33-39. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=182487



IEEE: Copy

SAFARIYOUN POUR, S., YAGHMAEI, S., GHOBADINEZHAD, Z., 2010. IMMOBILIZATION OF ALKALINE PROTEASE PRODUCED FROM BACILLUS LICHENIFORMIS PTCC1331 ON CALCIUM ALGINATE (ITS EFFECT ON ENZYMATIC ACTIVITY). JOURNAL OF CHEMICAL AND PETROLEUM ENGINEERING (JOURNAL OF FACULTY OF ENGINEERING), [online] 43(2), pp.33-39. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=182487.



 
 
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