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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   2010 , Volume 39 , Number 2; Page(s) 105 To 109.
 
Paper: 

PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE

 
 
Author(s):  SHAHBAZI A., MIRHENDI H., RAEISI A.*
 
* DISEASE MANAGEMENT CENTER, MINISTRY OF HEALTH, TEHRAN, IRAN
 
Abstract: 

Background: The importance of accurate diagnosis of all of major diseases cannot be underestimated and efficient laboratory testing is vital to identifying and treating life-threatening illnesses including malaria. In this study, we compared the potential of one of merozoite surface protein genes, PvMSP-3b, for detection of Plasmodium vivax in blood samples by PCR with routinely used marker, ssrRNA gene.
Methods: One hundred P. vivax microscopy-positive blood samples were simultaneously tested with two genetic markers, including PvMSP-3
b gene and ssrRNA gene by PCR and nestedPCR method, respectively, and their sensitivity and specificity in detection of P. vivax was compared.
Results: An important difference was seen in sensitivity between the 2 genetic markers, 100% in case of ssrRNA gene vs. 95% of PvMSP-3
b gene. The specificity of the two markers was same (100%). Microscopic diagnoses of thick and thin blood smears was used as "golden standard" method.
Conclusion: Due to critical importance of accurate detection of the parasite in malarious area, the PvMSP-3
b gene cannot be a suitable marker for detection of P. vivax in blood sample by PCR. More investigations are needed to find other valid markers.

 
Keyword(s): PVMSP-3β, PLASMODIUM VIVAX, SSRRNA, IRAN
 
 
References: 
 
Citations: 
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APA: Copy

SHAHBAZI, A., & MIRHENDI, H., & RAEISI, A. (2010). PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE. IRANIAN JOURNAL OF PUBLIC HEALTH, 39(2), 105-109. https://www.sid.ir/en/journal/ViewPaper.aspx?id=177130



Vancouver: Copy

SHAHBAZI A., MIRHENDI H., RAEISI A.. PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE. IRANIAN JOURNAL OF PUBLIC HEALTH. 2010 [cited 2021November30];39(2):105-109. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=177130



IEEE: Copy

SHAHBAZI, A., MIRHENDI, H., RAEISI, A., 2010. PLASMODIUM VIVAX MSP-3β GENE AS A GENETIC MARKER FOR THE PARASITE DETECTION IN COMPARISON WITH SSRRNA GENE. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 39(2), pp.105-109. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=177130.



 
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